MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

少数族裔博士前奖学金计划

• 批准号: 6704230
• 负责人: LAURA Benjamin NGWENYA
• 金额: $ 3.54万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-01 至 2005-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6704230
• 关键词: Macaca mulatta; age difference; astrocytes; behavior test; biological models; bromodeoxyuridine; cell differentiation; cell proliferation; cognition; computer program /software; confocal scanning microscopy; hippocampus; immunocytochemistry; learning; neural plasticity; neurogenesis; oligodendroglia; predoctoral investigator

DESCRIPTION (provided by applicant): Using a non-human primate model of normal aging, this investigation will explore the role of adult cell genesis in the hippocampus and surrounding areas of the primate brain. Bromodeoxyuridine (BrdU, a marker of cell proliferation) will be injected into both young and old rhesus monkeys (Macaca mulatta). After injection of BrdU, a subset of monkeys will be given a behavioral task to induce neurogenesis. Post-mortem analysis of brain tissue using immunohistochemistry, stereology, and confocal microscopy, will identify newly created neurons, astrocytes, and oligodendrocytes. This study will analyze two sets of experimental data. 1) Differentiation of newly created cells under basal conditions will be examined in young and old animals. Preliminary results suggest that there are changes in adult cell genesis with age; the details of these changes will be explored. 2) Learning has been shown to increase adult neurogenesis in the hippocampus. This plasticity will be compared in young and old animals that received post-BrdU behavioral testing. It has been established that cognitive decline occurs with age. This proposed investigation will determine if there are changes in adult cell genesis that could be important in the neurobiological basis of age-related cognitive decline.

描述（由申请人提供）：使用正常衰老的非人类灵长类动物模型，该研究将探讨成年细胞起源在灵长类动物大脑的海马及其周围区域中的作用。溴脱氧尿苷（BRDU，细胞增殖的标记）将被注入年轻人和老恒河猴（Macaca mulatta）中。注射BRDU后，将为一部分猴子赋予诱导神经发生的行为任务。使用免疫组织化学，立体学和共聚焦显微镜对脑组织进行验尸分析，将鉴定新产生的神经元，星形胶质细胞和少突胶质细胞。这项研究将分析两组实验数据。 1）在年轻动物和老年动物中将检查新创建的细胞的分化。初步结果表明，成年细胞发生随着年龄的增长而发生变化。这些更改的详细信息将探讨。 2）已显示学习可以增加海马中的成年神经发生。在接受BRDU后行为测试的年轻动物和老年动物中，将比较这种可塑性。已经确定认知能力下降随着年龄的增长而发生。该提出的研究将确定成年细胞起源的变化是否在与年龄相关的认知能力下降的神经生物学基础上可能很重要。