Gene expression in regenerating adult vertebrate CNS.

成年脊椎动物中枢神经系统再生中的基因表达。

• 批准号: 6837224
• 负责人: JUDITH A CHAPMAN
• 金额: $ 4.47万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-05 至 2007-06-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6837224
• 关键词: Chordata; Xenopus; acoustic nerve; behavior test; central nervous system; computer data analysis; cranial nerves; developmental genetics; fluorescent dye /probe; functional ability; gene expression; genetic regulation; mature animal; nervous system regeneration; neural plasticity; northern blottings; polymerase chain reaction; predoctoral investigator; sound perception; statistics /biometry; synaptogenesis; western blottings

DESCRIPTION (provided by applicant): Injury to the adult mammalian central nervous system (CNS) is usually permanent and progressive, and can be the result of disease or traumatic injury. Many recent studies have had limited success, possibly due to use of favorite models incapable of CNS regeneration. In order to further understand CNS regeneration in the adult, and to apply this knowledge on the clinical level, these factors must be elucidated in an adult model capable of regeneration in the absence of extraneous perturbations. Understanding what these factors are, and how they act to drive recovery, will allow further understanding of neural regeneration as a whole, and treatment options based on targeted flux through these pathways. This research training application involves adult Xenopus laevis, a vertebrate model capable of cranial nerve regeneration into the CNS, resulting in recovery of function of the vestibuloauditory nerve (nVIIl). The specific aims are: (1) discovery of genes driving regeneration in the adult vertebrate nVIII; (2) quantification of behavioral recovery of function; and (3) anatomical verification of nVIII lesion and extent of regeneration. Genes will be compared with those implicated in normal development and with models incapable of CNS regeneration.

描述（由申请人提供）：成年哺乳动物中枢神经系统（CNS）的损伤通常是永久性的和进行性的，并且可能是疾病或外伤的结果。最近的许多研究取得的成功有限，可能是由于使用了无法中枢神经系统再生的最喜欢的模型。为了进一步了解成人的中枢神经系统再生，并将这些知识应用于临床水平，必须在能够在没有外来扰动的情况下再生的成人模型中阐明这些因素。了解这些因素是什么，以及它们如何促进恢复，将有助于进一步了解整个神经再生，以及基于这些途径的目标通量的治疗方案。这项研究训练应用涉及成年非洲爪蟾，这是一种能够将脑神经再生到中枢神经系统的脊椎动物模型，从而恢复前庭听觉神经（nVIIl）的功能。具体目标是：（1）发现驱动成年脊椎动物nVIII再生的基因； (2)行为功能恢复的量化； (3) nVIII 损伤和再生程度的解剖学验证。基因将与正常发育相关的基因以及无法中枢神经系统再生的模型进行比较。