Early Host Response Elicitation by Agrobacterium

农杆菌诱导早期宿主反应

• 批准号: 6791376
• 负责人: MARION L BRODHAGEN
• 金额: $ 0.75万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-16 至 2004-10-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6791376
• 关键词: Agrobacterium tumefaciens; Arabidopsis; Escherichia coli; Pseudomonas; bacterial genetics; gene expression; gene induction /repression; gene mutation; genetic transcription; host organism interaction; microarray technology; plant genetics; polymerase chain reaction; postdoctoral investigator; tissue /cell culture

DESCRIPTION (provided by applicant): The recent availability of genome sequence and microarray technology is bringing about a profound expansion in our level of understanding of host-microbe interactions. The proposed study will utilize a pathosystem in which both the pathogen and host are well characterized and genetically tractable, and whose genomes are fully sequenced. Early host responses of Arabidopsis thaliana to the unique pathogen and natural genetic engineer Agrobacterium tumefaciens will be investigated at the level of gene expression, taking advantage of newly available, comprehensive oligonucleotide microarrays representing all of the approximately 26,000 known and predicted open reading frames for Arabidopsis. Transcriptional changes elicited in Arabidopsis by Agrobacterium will be compared to those elicited by two other, dissimilar bacteria (the nonpathogenic E. coil and typical plant pathogen, Pseudomonas syringae) to identify plant responses unique to Agrobacterium challenge. Bacterial gene(s) which, in turn, are required to elicit the host response, will be identified. The insights gained from such a study will i) contribute to the understanding of eukaryote-bacteria interactions; ii) contribute information useful for exploiting Agrobacterium the natural genetic engineer, whose host range has been expanded recently to include fungi, yeast, and even mammalian cells, but is not universal, and iii) provide a basis for comparison of host responses to phylogenetically proximal members of the alpha-2 proteobacteria, which include plant symbionts and mammalian pathogens.

描述（由申请人提供）：最近基因组序列和微阵列技术的可用性正在深刻扩展我们对宿主-微生物相互作用的理解水平。拟议的研究将利用一个病理系统，其中病原体和宿主均已被充分表征且在遗传上易于处理，并且其基因组已完全测序。拟南芥对独特病原体和天然基因工程农杆菌的早期宿主反应将在基因表达水平上进行研究，利用代表拟南芥所有约 26,000 个已知和预测开放阅读框的新可用的综合寡核苷酸微阵列。将农杆菌在拟南芥中引起的转录变化与其他两种不同细菌（非致病性大肠杆菌和典型植物病原体丁香假单胞菌）引起的转录变化进行比较，以确定植物对农杆菌挑战的独特反应。将鉴定引发宿主反应所需的细菌基因。从此类研究中获得的见解将 i) 有助于理解真核生物-细菌相互作用； ii) 提供对利用天然基因工程师农杆菌有用的信息，其宿主范围最近已扩大到包括真菌、酵母，甚至哺乳动物细胞，但并不普遍，以及 iii) 为比较宿主对系统发育近端的反应提供基础α-2 变形菌门的成员，包括植物共生体和哺乳动物病原体。