Role of the SasAB locus in S. aureus Biofilms

SasAB 基因座在金黄色葡萄球菌生物膜中的作用

• 批准号: 6834009
• 负责人: ROBERT M SHANKS
• 金额: $ 4.73万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6834009
• 关键词: Staphylococcus aureus; bacteria infection mechanism; bacterial genetics; bacterial proteins; biofilm; gene expression; genetic transcription; microorganism metabolism; polymerase chain reaction; postdoctoral investigator; protein biosynthesis; protein localization; protein structure function; regulatory gene; site directed mutagenesis; southern blotting; tissue /cell culture; transfection /expression vector; virulence

DESCRIPTION (provided by applicant): Biofilms are communities of bacteria that adhere to surfaces, and represent the physiological state in which most bacteria exist. Bacteria in biofilms behave differently than do bacterial cells suspended in culture medium (planktonic cells). Notably cells in biofilms are much more resistant to antibiotics and other biocides. Biofilms profoundly affect mankind. There are many links between biofilms and disease; it is estimated that more than half of all human bacterial infections involve biofilms. Biofilms growing on tissue, implants and medical tubing can be highly problematic and extremely difficult to treat. Staphylococcus aureus is major agent of nosocomial infections and capable of forming robust biofilms on biotic and abiotic surfaces. Yet, we know only a limited number of factors that contribute to S. aureus biofilm formation. Identified factors may become important vaccine and drug targets. Therefore, it will be important to elucidate the genetic factors involved in S. aureus biofilm formation. This proposal describes biochemical and genetic experiments designed to characterize novel factors that contribute to S. aureus biofilm formation.

描述（由申请人提供）：生物膜是粘附在表面的细菌群落，代表大多数细菌存在的生理状态。生物膜中的细菌的行为与悬浮在培养基中的细菌细胞（浮游细胞）不同。值得注意的是，生物膜中的细胞对抗生素和其他杀菌剂的抵抗力要强得多。生物膜深刻地影响着人类。生物膜与疾病之间存在许多联系；据估计，超过一半的人类细菌感染涉及生物膜。组织、植入物和医用管道上生长的生物膜可能会带来很大的问题，并且极难治疗。金黄色葡萄球菌是医院感染的主要病原体，能够在生物和非生物表面形成坚固的生物膜。然而，我们只知道促进金黄色葡萄球菌生物膜形成的有限因素。已确定的因素可能成为重要的疫苗和药物靶点。因此，阐明金黄色葡萄球菌生物膜形成中涉及的遗传因素非常重要。 该提案描述了旨在表征有助于金黄色葡萄球菌生物膜形成的新因素的生化和遗传实验。

项目成果

Catheter lock solutions influence staphylococcal biofilm formation on abiotic surfaces.

• DOI: 10.1093/ndt/gfl170
• 发表时间: 2006-08
• 期刊: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
• 作者: R. Shanks;J. Sargent;R. M. Martínez;Martha L. Graber;G. O’Toole