Human Pathogens and Coastal Ocean Processes

人类病原体和沿海海洋过程

• 批准号: 6747098
• 负责人: REBECCA J GAST
• 金额: $ 8.14万
• 依托单位: WOODS HOLE OCEANOGRAPHIC INSTITUTION
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2008-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6747098
• 关键词: Acanthamoeba; Cryptosporidium; Giardia; Legionella; Naegleria; Protista; Sarcodina; United States; Vibrio; aquatic organism; bacteria; denaturing gradient gel electrophoresis; disease reservoirs; fluorescent in situ hybridization; geographic site; in situ hybridization; marine toxins; microorganism population study; polymerase chain reaction; public health; saltwater environment; sample collection; sedimentation; temperature; water microbiology; water quality; water sampling /testing

Some human pathogens are natural members of the marine microbial community (e.g Vibrio species), but many others, such as the protist Giardia, are potentially introduced into seawater from sewage input and terrestrial runoff. Despite the potential for their impact on human health, relatively little is known about the occurrence, much less the prevalence and distribution, of traditionally non-marine human pathogens. The survival and persistence of various pathogens is most certainly influenced by environmental conditions, and the contamination of coastal and estuarine areas through freshwater runoff, sewage and thermal pollution have the potential to alter local environmental conditions. Studies that examine pathogen presence and persistence need to be coupled with predictive models in order to begin to determine where and when the risks of potential infection, or simply the presence of pathogens, are highest. Our project aims are to use molecular methodology to determine the distribution and abundances of the human pathogens (including Giardia, Cryptosporidium, Acanthamoeba, Naegleria, Vibrio and LegioneIla), determine whether naturally occurring marine amoebae can serve as reservoirs for pathogenic bacteria, and look for evidence of natural occurrence of these associations in the anthropogenically impacted estuarine environment of Mount Hope Bay, MA. Data will be collected over spatially and temporally relevant scales (multiple sites, multiple years). Information on the physico-chemical characteristics will be correlated with biological distribution and persistence information for use in evaluating a water quality/eutrophication model for the bay that could be used to predict where and when pathogens could be found.

一些人类病原体是海洋微生物群落（例如颤音物种）的自然成员，但是许多其他人的病原体，例如原则贾第鞭毛虫，可能会从污水输入和陆地径流中引入海水。尽管对人类健康的影响有潜力，但对传统非陆地人类病原体的发生，更不用说的发生率和分布相对较少。各种病原体的生存和持久性肯定会受到环境条件的影响，并且通过淡水径流，污水和热污染对沿海和河口区域的污染有可能改变当地的环境条件。检查病原体存在和持久性的研究需要与预测模型相结合，以便开始确定何时何地 潜在感染的风险或简单的病原体存在最高。 Our project aims are to use molecular methodology to determine the distribution and abundances of the human pathogens (including Giardia, Cryptosporidium, Acanthamoeba, Naegleria, Vibrio and LegioneIla), determine whether naturally occurring marine amoebae can serve as reservoirs for pathogenic bacteria, and look for evidence of natural occurrence of these associations in the anthropogenically impacted estuarine马萨诸塞州希望山山的环境。数据将在空间和时间相关的量表上（多个地点，多年）收集。有关物理化学特征的信息将与生物分布和持久性信息相关，以评估海湾的水质/富营养化模型，该模型可用于预测可以找到病原体的何时何时以及何时发现病原体。