600 MHz NMR Spectrometer

600 MHz 核磁共振波谱仪

• 批准号: 6580257
• 负责人: MARK E. GIRVIN
• 金额: $ 50万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580257
• 关键词: biomedical equipment purchase; nuclear magnetic resonance spectroscopy; structural biology

DESCRIPTION (provided by applicant): Albert Einstein College of Medicine inaugurated a Structural NMR Resource in 1995 with the hire of a new faculty member (Girvin), and the purchase of an initial four-channel Bruker DRX 600 MHz instrument (delivered late 1996) equipped for determining the structures and dynamics of biological macromolecules. This shared NMR Resource has been an enormous success. The PI has developed a program in the NMR structural analysis of membrane proteins, which has attracted collaborators from other institutions. Equal effort went into assisting other AECOM faculty incorporates NMR into their research programs. These efforts have been extremely productive, with many active, well-funded independent investigators including NMR-intensive Specific Aims in their current NIH and NSF grants. Due to these efforts, usage has grown to where instrument demand exceeds the capacity of our single 600 - by a factor of 2.5. We are requesting partial funding for a $1,026,900 Bruker DRX 600 MHz spectrometer with cryoprobe ($500,000 from the NIH, $200,000 from the NSF, with the remainder coming from school funds). This instrument will enable the participating group of independent investigators to accomplish the specific aims of their NIH and NSF funded proposals. The projects fall into four broad areas: i) membrane protein structure and function (Investigators Girvin, Spray, Almo, Krulwich, and Cramer), ii) protein folding and dynamics (Investigators Callender, Grubmeyer, and Girvin), iii) enzyme mechanism, regulation, and structure (Investigators Backer, Schramm, Blanchard, and Grubmeyer), and immunology (Investigators Nathenson and Almo).

描述（由申请人提供）：阿尔伯特·爱因斯坦医学院于 1995 年启用了结构 NMR 资源，聘用了一名新教员 (Girvin)，并购买了最初的四通道 Bruker DRX 600 MHz 仪器（1996 年末交付）配备用于确定生物大分子的结构和动力学。 这一共享 NMR 资源取得了巨大成功。 PI 开发了一个膜蛋白核磁共振结构分析程序，吸引了其他机构的合作者。我们同样努力帮助其他 AECOM 教师将 NMR 纳入他们的研究项目。这些努力非常富有成效，许多活跃的、资金充足的独立研究人员在他们当前的 NIH 和 NSF 拨款中包括 NMR 密集型特定目标。由于这些努力，使用量已经增长到仪器需求超过我们单个 600 的容量的 2.5 倍。 我们正在请求部分资金用于购买价值 1,026,900 美元的带冷冻探头的 Bruker DRX 600 MHz 光谱仪（NIH 提供 500,000 美元，NSF 提供 200,000 美元，其余部分来自学校基金）。该工具将使参与的独立研究人员小组能够实现其 NIH 和 NSF 资助的提案的具体目标。这些项目分为四大领域：i) 膜蛋白结构和功能（研究人员 Girvin、Spray、Almo、Krulwich 和 Cramer），ii) 蛋白质折叠和动力学（研究人员 Callender、Grubmeyer 和 Girvin），iii) 酶机制，调节、结构（研究人员 Backer、Schramm、Blanchard 和 Grubmeyer）以及免疫学（研究人员 Nathenson 和 Almo）。