Purchase of Bruker Pulse EPT Spectrometer

购买布鲁克脉冲EPT光谱仪

• 批准号: 6580583
• 负责人: DAVID S CAFISO
• 金额: $ 50万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580583
• 关键词: biomedical equipment purchase; electron spin resonance spectroscopy; membrane proteins; protein structure function; protein transport

DESCRIPTION (provided by applicant): Funds are requested to purchase a Bruker Elexsys 580 EPR spectrometer. This is a highly versatile commercial EPR machine that will be capable of both CW operation as well as a broad range of pulse experiments with ns time resolution. Options are requested that will enable saturation recovery as well as ELDOR experiments. This will be a multiuser instrument that will be housed and maintained by the Department of Chemistry. The primary user group will consist of five research groups at the University of Virginia: Profs. Bryant and Cafiso in the Department of Chemistry and Profs. Perozo, Tamm and Nakamoto in the Department of Molecular Physiology. The facility will also be available to other research groups at the University. This user group presently makes heavy use of the existing CW EPR capabilities at the University of Virginia, however, there are presently no instruments on campus or nearby that are capable of performing pulse EPR measurements. The work being carried out by most users in this group involves site-directed spin labeling to determine membrane protein structure and dynamics. One group is using paramagnetic species (such as nitroxides) to produce nuclear relaxation enhancements and determine dynamics in proteins. Compared to CW experiments, pulse EPR experiments enable a direct determination of electron spin-lattice relaxation rates and exchange between between spins. Methods to determine dipolar coupling between spins and estimate interspin distances can be extendent to much longer distances using pulse methods and do not require the acquisition of non-interacting reference spectra. In addition, the pulse experiment can eliminate problems due to orientational anisotropy in the CW experiment. This is user group will use pulse EPR to provide information on the structures and structural changes in proteins that facilitate transport across biological membranes, the structures and mechanisms of proteins that facilitate membrane attachment in cell signaling systems and the mechanisms of protein-mediated membrane fusion.

描述（由申请人提供）：要求资金购买Bruker Elexsys 580 EPR光谱仪。这是一款通用性的商业EPR机器，可以同时进行CW操作以及NS时间分辨率的广泛脉冲实验。请求选项，以实现饱和恢复以及Eldor实验。这将是一种多源工具，该工具将由化学系载和维护。主要用户组将由弗吉尼亚大学的五个研究小组组成：教授。化学和教授系的科比和Cafiso。分子生理系的perozo，tamm和nakamoto。该设施也将提供给大学的其他研究小组。该用户组目前大量利用弗吉尼亚大学现有的CW EPR功能，但是，目前校园或附近没有能够执行脉冲EPR测量的乐器。该组中大多数用户进行的工作涉及定向的自旋标记，以确定膜蛋白结构和动力学。一组正在使用顺磁性物种（例如硝基氧化物）产生核弛豫增强并确定蛋白质的动力学。与CW实验相比，Pulse EPR实验可以直接确定电子自旋晶格松弛率和两次旋转之间的交换。确定旋转和估算速旋距离之间的偶极耦合的方法可以使用脉冲方法扩展到更长的距离，并且不需要获取非相互作用的参考光谱。另外，由于CW实验中的定向性各向异性，脉冲实验可以消除问题。这是用户组使用脉冲EPR来提供有关蛋白质的结构和结构变化的信息，这些信息促进了跨生物膜的传输，蛋白质的结构和机制，这些蛋白质和机制促进了细胞信号系统中膜附着的膜附着以及蛋白质介导的膜融合的机制。