Diagnostic and pathogeneic investigations of vaccinia vi

痘苗病毒的诊断和病原学研究

• 批准号: 6679910
• 负责人: INDIRA HEWLETT
• 金额: --
• 依托单位: BUREAU OF HEALTH PLANNING AND RESOURCES DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6679910
• 关键词: active immunization; blood disorder diagnosis; cytotoxic T lymphocyte; diagnosis design /evaluation; diagnosis quality /standard; immunity; plasma; tissue /cell culture; vaccinia virus; viral vaccines; virus cytopathogenic effect; virus load

Summary: We have initiated studies to investigate the period of viremia post-vaccination. Cells and plasma from recent vaccinnes enrolled in candidate vaccine trials are being collected at various time points 0, 3, 7, 14, 28 and 56 days. Sensitive Taqman and virus culture assays are being developed. In addition, currently licensed viral marker tests used to screen donors will be evaluated for potential cross-raectivity of interference with samples from immunized individuals. We are also investigating CTL epitopes involved in the immune response against vaccinia.

摘要：我们已经开始研究疫苗接种后病毒血症期。在不同时间点0、3、7、14、28和56天，正在收集候选疫苗试验中最近疫苗试验的疫苗的细胞和血浆。 敏感的塔克曼和病毒培养试验正在开发。此外，将评估目前用于筛选供体的持有许可的病毒标记测试，以评估潜在的交叉偏移性与免疫个体样品的干扰。 我们还正在研究涉及疫苗免疫反应的CTL表位。