lopment And Diagnostic Use Of Rapid Immunoassays

快速免疫分析的配置和诊断用途

• 批准号: 6675214
• 负责人: Alan Thomas Remaley
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6675214
• 关键词: biomarker; biotechnology; breast neoplasm /cancer diagnosis; diagnosis design /evaluation; diagnostic tests; gastrins; human tissue; immunologic assay /test; patient oriented research; rapid diagnosis; technology /technique development; biomarker; biotechnology; breast neoplasm /cancer diagnosis; diagnosis design /evaluation; diagnostic tests; gastrins; human tissue; immunologic assay /test; patient oriented research; rapid diagnosis; technology /technique development

Several endocrine tumor markers are routinely used in the diagnosis and management of various cancers. The current assays, however, typically take several hours to perform, which precludes their use in the intraoperative management of patients. Most endocrine tumor markers have a half-life in the circulation of less than 5 minutes, thus making it feasible, if a rapid assay were available, for monitoring the concentration of the hormones during surgery or localization procedures. The primary indication for such assays would be to assess the extent of residual tumor after surgery and to localize tumors by selective venous or arterial sampling. In the past year, we have completed the development of a new intact PTH assay that does not cross react with the 7-84 fragment of PTH. We have compared the old and new rapid PTH assay and have found that there is faster decay of PTH with the new assay following parathyroidectomy. We have also completed an analysis of the possible diagnostic utilty of a rapid ACTH assay on jugular venous samples. The results showed that such an assay would be useful for preventing the need for petrosal venous sampling in about 50% of the cases for Cushing's disease. In the upcoming year, we plan to further develop a rapid gastrin assay for localizing gastrinomas.

通常将几种内分泌肿瘤标记用于各种癌症的诊断和管理。但是，目前的测定通常需要几个小时才能进行，这排除了他们在患者的术中治疗中的使用。大多数内分泌肿瘤标记物在不到5分钟的循环中具有半衰期，因此，如果可以进行快速测定，则可以在手术或定位程序中监测激素的浓度。此类测定法的主要指示是评估手术后残留肿瘤的程度，并通过选择性静脉或动脉采样来定位肿瘤。在过去的一年中，我们已经完成了一种新完整的PTH测定法的开发，该测定法不会与PTH的7-84片段反应。我们已经比较了旧的和新的快速PTH分析，发现PTH与甲状旁腺切除术后的新测定法更快。我们还完成了对颈静脉样品中快速ACTH分析的可能诊断效应的分析。结果表明，这种测定对于防止大约50％的库欣氏病例中的质子静脉抽样非常有用。在接下来的一年中，我们计划进一步开发一种胃病快速分析，以定位胃瘤。