Development and Investigation of Assays For Cardiovascular Risk Assessment

心血管风险评估检测方法的开发和研究

• 批准号: 8565337
• 负责人: Alan Thomas Remaley
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8565337
• 关键词: Apolipoproteins B; Biological Assay; Cardiovascular system; Categories; Cholesterol; Clinical; Coronary Arteriosclerosis; Data Analyses; Development; Dyslipidemias; Effectiveness; Equation; Event; Gold; High Density Lipoproteins; Investigation; Laboratories; Lipoproteins; Low-Density Lipoproteins; Measures; Methods; Patients; Performance; Population; Risk Assessment; Risk Estimate; Testing; Ultracentrifugation; Work; base; cardiovascular risk factor; diagnostic accuracy; improved; physical separation

Direct HDL-C and LDL-C methods, which do not require the physical separation of lipoproteins, offer many practical advantages for routine clinical laboratory testing and are now widely utilized. Based on our previous work, we found, however, that they often yield results that substantially differ by the gold standard method, involving ultracentrifugation. In the past year, we have extended this work by assessing the impact of analytical errors in the calculation of Non-HDL-C in cardiovascular risk assessment. The results show that for patients with a dyslipidemia that Non-HDL-C, using a direct HDL-C assay, was superior to using a direct LDL-C in categorizing patients into cardiovascular risk categories. We also found that for some direct HDL-C assays that alternatives to the Friedewald equation for calculating LDL-C showed better correspondence to ultracentrifugation. We also completed a study and are now analyzing the data comparing apoB to LDL-P, as measured by NMR, in predicting cardiovascular events in the MESA population.

不需要物理分离脂蛋白的直接HDL-C和LDL-C方法为常规临床实验室测试提供了许多实际优势，并且现在已广泛使用。 但是，根据我们以前的工作，我们发现它们通常会产生的结果，这些结果通过黄金标准方法涉及超速离心。 在过去的一年中，我们通过评估分析错误在非HDL-C计算心血管风险评估中的计算中的影响来扩展这项工作。 结果表明，对于血脂异常的患者，使用直接HDL-C分析的非HDL-C优于使用直接LDL-C将患者分类为心血管风险类别。 我们还发现，对于一些直接的HDL-C分析，用于计算LDL-C的Friedewald方程的替代方案显示出与超速离心的更好的对应。 我们还完成了一项研究，现在正在分析NMR测量的APOB与LDL-P进行比较的数据，以预测MESA种群中的心血管事件。