MOLECULAR EVOLUTION OF SEX DETERMINATION

性别决定的分子进化

• 批准号: 6489935
• 负责人: Mark L Siegal
• 金额: $ 4.62万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6489935
• 关键词: Drosophilidae; arthropod genetics; biochemical evolution; gene dosage; gene expression; gene interaction; genetic polymorphism; molecular biology; molecular cloning; polymerase chain reaction; restriction fragment length polymorphism; sex behavior; sex determination; sex differentiation; southern blotting

The long-term objective of the proposed research is to understand the functional divergence and conservation of the sex-determination pathway in flies and other taxa. The first specific aim is to test the hypothesis that those components of the pathway that act last in the hierarchy are most likely to be conserved. The second specific aim is to study the evolutionary acquisition of molecular function. The third specific aim is to test competing hypothesis about the expected level of within species variation ingenues acting near the beginning of the sex-determination hierarchy, as compared with the level of within species variation in genes acting near the end of the hierarchy. The value of this research is that it will illuminate the process by which genetic pathways involve, and thereby produce a conceptual framework for applying knowledge of model systems like Drosophila to the development of the other organisms, including humans. The first two specific aims will be achieved by cloning and characterizing homologies of the intersex and determining mechanisms. To achieve the third specific aim, a mutation in hermaphrodite will be introgressed into a diverse set of wildtype fly strains, to uncover genetic variation in interacting genes involved in sex determination.

拟议研究的长期目标是了解果蝇和其他类群性别决定途径的功能差异和保守性。第一个具体目标是检验这样的假设：在层次结构中最后起作用的那些通路成分最有可能被保守。第二个具体目标是研究分子功能的进化获得。第三个具体目标是测试关于在性别决定层次结构开始附近起作用的物种内变异基因的预期水平的竞争假设，与在层次结构末端附近起作用的基因的物种内变异水平进行比较。这项研究的价值在于，它将阐明遗传途径所涉及的过程，从而产生一个概念框架，将果蝇等模型系统的知识应用于包括人类在内的其他生物体的发育。前两个具体目标将通过克隆和表征双性和确定机制的同源性来实现。为了实现第三个具体目标，雌雄同体的突变将被渗入到一组不同的野生型果蝇品系中，以揭示参与性别决定的相互作用基因的遗传变异。