Studies on Cryptosporidium parvum Type 1

小隐孢子虫 1 型的研究

• 批准号: 6757254
• 负责人: SAUL r TZIPORI
• 金额: $ 39.63万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-15 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6757254
• 关键词: Cryptosporidium; Primates; clinical research; complementary DNA; cow; cryptosporidiosis; egg /ovum; gene expression; genetic recombination; genetic strain; genotype; germ free condition; host organism interaction; human tissue; life cycle; microorganism culture; parasitic diseases; phenotype; protozoal genetics; restriction fragment length polymorphism; subtraction hybridization; swine; transmission electron microscopy

DESCRIPTION (Provided by the applicant): This is a revised proposal (R01AI150471-01) in response to PA 99-124. The goal of this project is a comprehensive characterization of Cryptosporidium parvum type 1, the most common agent of human cryptosporidiosis in the general population and in people with AIDS. Because until recently type 1 C. parvum could not be propagated in the laboratory, this subgroup is rarely studied and remains poorly characterized. As a consequence, phenotypic properties of calf-propagated type 2 oocysts are extrapolated to the whole species. In light of the significant public health importance of cryptosporidiosis caused by type 1 C. parvum, the emphasis of this proposal is twofold: 1) to extensively characterize C. parvum type 1 with respect to genotypic and phenotypic properties, as well as its life cycle and interaction with the host, and (2) to compare C. parvum type 1 and type 2 with the aim of gaining a better understanding of the species C. parvum and the disease caused by this parasite. The recent development of a gnotobiotic pig model suitable for propagation of type 1 and type 2 will facilitate the study of several type 1 isolates and the comparison of standardized C. parvum isolates of both types originating from the same host. The interaction of type 1 and type 2 isolates in mixed infections will also be studied in the pig model. Type 1 isolates included in this study will originate from chronic and acute infections and from different geographical areas. The study includes three specific aims: the first aim is a comprehensive analysis of ten type 1 C. parvum isolates. In the second aim the host-parasite interaction of type 1 and type 2 isolates will be compared using the pig model and cell culture models. The third aim investigates the extent of genetic variation and exchange within and between the two types of C. parvum to determine whether they belong to one or two species.

描述（由申请人提供）：这是一个修订的建议 （R01AI150471-01）响应PA 99-124。这个项目的目标是 1型隐孢子虫的全面表征，最多 人类和人口中人类隐孢子虫病的共同药物 与艾滋病。因为直到最近1型C. Parvum才能传播 实验室，该亚组很少研究，并且保持较差 特征。结果，小牛传播类型的表型特性 2个卵囊被外推到整个物种。鉴于重要 由1型C. Parvum引起的隐孢子虫病的公共卫生重要性， 该提议的重点是双重的：1）广泛表征C. parvum 关于基因型和表型特性及其寿命的类型1 与宿主的循环和相互作用，（2）比较C. parvum 1型和 2型旨在更好地了解物种C. parvum 以及该寄生虫引起的疾病。最近的发展 适用于1型和2型传播的gnotobiotic猪模型将 促进对几种1型分离株的研究和比较 两种类型的标准化C. parvum分离株，源自同一主机。 混合感染中类型1和2型分离株的相互作用也将是 在猪模型中进行了研究。本研究中包含的1型分离株将起源 从慢性和急性感染以及不同地理区域。这 研究包括三个具体目标：第一个目的是全面分析 十种1型C. parvum分离株。在第二个目标中，主寄生虫 使用猪模型将比较1型和2型分离株的相互作用 和细胞培养模型。第三个目标研究了遗传的程度 两种类型的C. parvum内和之间的变化和交换 确定它们是否属于一个或两个物种。