Core 3 - Bioanalytical Core

核心 3 - 生物分析核心

• 批准号: 6880823
• 负责人: Dennis McKenna
• 金额: $ 15.04万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6880823
• 关键词: Basidiomycetes; alternative medicine; analytical chemistry; biomedical facility; cooperative study; immunomodulators

The Bioanalytical Core will serve a four-fold function within the DCRC: 1) Core facility for assessing levels of immunopotentiating bioactive components of Trametes versicolor (Tv) and in tissues and serum provided by the three Projects; 2) Storage and distribution facility for the Tv extracts and control test compounds to all of the projects; 3) Central facility for beta-glucan fractionation, enzymatic degradation, and labeling; and 4).Training facility to learn botanical bioanalysis. The Core will be located at the University of Minnesota and lead by Dennis McKenna, PhD, a leading expert on mushrooms. Dr. McKenna will be responsible for performing all analyses performed within the Core. He will receive extracts from Paul Stamets and Gary Ostroff, PhD, maintain chain of custody On all test products and assess stability at regular intervals. He will work with the Minnesota Cellular and Cellular therapeutics facility to ensure quality control and purity of all Tv extracts as well as creation of a lyophilized Tv extract for clinical trials in the DCRC. Dr. Ostroff, an internationally recognized leader in beta-glucan research will serve as a consultant working with Dr. McKenna to a) fractionate, isolate and purify beta-glucan fractions from complex Tv extracts; b) enzymatically degrade beta-glucans in Tv to create a test compound for use in Projects 1 and 2; and c) create a fluorescently labeled purified beta-glucan for use in biodistribution studies.. The core will greatly benefit from the availability of high quality instrumentation, these resources, coupled with expert consultants will allow us to meet the analytical challenges posed by the Project Leaders and perform their research aims within the DCRC.

生物分析核心将在 DCRC 内发挥四重功能： 1) 评估三个项目提供的栓菌 (Tv) 以及组织和血清中免疫增强生物活性成分水平的核心设施； 2) 所有项目的电视提取物和控制测试化合物的储存和分配设施； 3) β-葡聚糖分馏、酶降解和标记的中央设施； 4).学习植物生物分析的培训设施。该核心将设在明尼苏达大学，由蘑菇领域的顶尖专家 Dennis McKenna 博士领导。 McKenna 博士将负责执行核心内进行的所有分析。他将收到保罗·斯塔梅茨和加里的摘录 奥斯特罗夫博士负责维护所有测试产品的监管链并定期评估稳定性。他将与明尼苏达细胞和细胞治疗机构合作，确保所有电视提取物的质量控制和纯度，并创建用于 DCRC 临床试验的冻干电视提取物。 Ostroff 博士是国际公认的 β-葡聚糖研究领域的领导者，他将担任顾问，与 McKenna 博士合作：a) 从复杂的电视提取物中分馏、分离和纯化 β-葡聚糖组分； b) 酶促降解 Tv 中的 β-葡聚糖，以创建用于项目 1 和 2 的测试化合物； c) 创建荧光标记的 用于生物分布研究的纯化β-葡聚糖。核心将极大地受益于高质量仪器的可用性，这些资源加上专家顾问将使我们能够应对项目负责人提出的分析挑战，并在范围内实现他们的研究目标发展研究中心。