Core--Cell culture

核心--细胞培养

• 批准号: 6869588
• 负责人: MONTY KRIEGER
• 金额: $ 21.64万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-12-10 至 2005-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6869588
• 关键词: biomedical facility; blood lipoprotein; fluorescence microscopy; monoclonal antibody; receptor binding; stem cells; tissue /cell culture

The Cell Culture Core facility will provide equipment (e.g., workstations, storage facilities and specialized microscopes) and material technical support for all of the projects. For example, it will maintain our collection of totipotent stem cells and houses or large scale cell culture facility for production of recombinant DNA-derived and naturally occurring proteins. The facility will generate large numbers of cells for those projects requiring the isolation of cellular organelles of other cell products (e.g., transcription factors), and provide advice and material assistance in the isolation/purification of the cell products. It will serve as a center for fluorescence (standard, inverted and confocal microscopes are available) and for the generation of both rat and mouse monoclonal antibodies. It will also be a source of native and modified lipoproteins and lipoprotein-deficient sera, FPLC analysis of serum lipoprotein profiles, special media formulations, mediating testing, and facilities for use of potentially pathogenic reagents. The facility will also provide a high sensitivity Kodak Image Station 440 CF and 1-D Image Analysis software for immunoreceptor assays, in which the quantitative of ligand binding to receptors and transporters can be readily measured. All of the individual of the Program employ animal cell culture techniques. In some cases of the cultured cells represent a primary experimental system, while in oth4ers these cells are sources of regents or of intermediates used for the generation of animal models. The goal of the Cell Culture Core is to facilitate research individual projects and to foster collaborative studies by providing specialized facilities and material and technical support to the Program.

细胞培养核心设施将为所有项目提供设备（例如，工作站，存储设施和专业显微镜）和物质技术支持。例如，它将保持我们的全能干细胞和房屋或大规模细胞培养设施的收集，以生产重组DNA衍生和自然存在的蛋白质。该设施将为需要隔离其他细胞产品（例如转录因子）的细胞细胞器的项目生成大量细胞，并在分离/纯化细胞产品的情况下提供建议和物质辅助。它将用作荧光的中心（标准，倒置和共聚焦显微镜），并用于生成大鼠和小鼠单克隆抗体。它也将是天然和改良的脂蛋白和缺乏蛋白质的血清的来源，血清脂蛋白谱的FPLC分析，特殊的培养基配方，中介测试以及使用潜在病原试剂的设施。该设施还将提供高灵敏度柯达图像台440 CF和1-D图像分析软件，用于免疫受体测定，其中可以轻松地测量配体结合与受体和转运蛋白的定量。该程序的所有个体都采用动物细胞培养技术。在某些情况下，培养的细胞代表了主要的实验系统，而在OTH4ER中，这些细胞是用于生成动物模型的摄政源或中间体的来源。细胞培养核心的目的是通过为该计划提供专门的设施，材料和技术支持来促进研究个体项目并促进协作研究。