Analysis of the Drosophila S2 cell cytoskeleton

果蝇 S2 细胞骨架分析

• 批准号: 6836274
• 负责人: REED J KELSO
• 金额: $ 3.8万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2005-06-10
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6836274
• 关键词: Drosophilidae; RNA interference; actins; arthropod genetics; cell membrane; cell migration; cellular polarity; cytoskeleton; fluorescence microscopy; gene induction /repression; genetic library; microfilaments; postdoctoral investigator; protein transport; tissue /cell culture; transmission electron microscopy

DESCRIPTION (provided by applicant): The dynamics of the actin network at the leading edge of a cell are used to produce the mechanical forces necessary for cell migration. Because the cell membrane is the site where many of the proteins implicated in the regulation of retrograde flow are localized it is of particular interest to focus on retrograde flow solely at the cell membrane. All of the current knowledge about the dynamics of retrograde flow is known from observations of retrograde flow occurring in the cytoplasm, leaving unexplored any of the dynamic interactions between the actin network and the cell membrane. This proposal seeks to use total internal reflection fluorescence (TIRF) microscopy and transmission electron microscopy (TEM), combined with RNA interference, in Drosophila S2 cells to investigate retrograde flow, specifically at the cell membrane.

描述（由申请人提供）： 细胞前缘肌动蛋白网络的动力学用于产生细胞迁移所需的机械力。由于细胞膜是许多参与逆行流调节的蛋白质定位的部位，因此仅关注细胞膜上的逆行流特别令人感兴趣。目前关于逆行流动力学的所有知识都是通过对细胞质中发生的逆行流的观察而得知的，而肌动蛋白网络和细胞膜之间的动态相互作用尚未被探索。该提案试图在果蝇 S2 细胞中使用全内反射荧光 (TIRF) 显微镜和透射电子显微镜 (TEM) 并结合 RNA 干扰来研究逆行流，特别是在细胞膜上。