Integration of Chemosensory and Hormonal Stimuli

化学感应和激素刺激的整合

• 批准号: 6693404
• 负责人: RUTH I. WOOD
• 金额: $ 24.38万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-15 至 2006-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6693404
• 关键词: Integration; Chemosensory; Hormonal; Stimuli

DESCRIPTION (provided by applicant): Gonadal steroid hormones are essential for mating in male hamsters, as in most mammals. Castration also reduces sexual motivation, as measured by interest in vaginal odors and preference for a receptive female. However, understanding of mechanisms for steroid stimulation of appetitive sexual behavior is limited. This is the overall objective of the proposed studies. Hamsters are an important model for investigating sexual motivation because mating is dependent on chemosensory cues. Male hamsters engage in extensive anogenital investigation of females before mating, and removal of the olfactory bulbs abolishes both copulation and preference for an estrous female. Studies in Specific Aim 1 will investigate the effects of castration and steroid replacement on detection of and responsiveness to female hamster vaginal secretion (FHVS) to determine if castrated males can detect FHVS, and the steroid signals that convey responsiveness to FHVS. Conditioned taste avoidance will be used to assess chemosensory detection (Aim 1A), while Aims 1B and 1C will evaluate chemosensory responsiveness using operant responding for FHVS. Specific Aim 2 will determine brain pathways for hormonal control of chemosensory responsiveness. Aim 2A will investigate the transduction of rewarding chemosensory cues in the olfactory mucosa and/or vomeronasal organ. Aims 2B and 2C will focus on central targets of the olfactory bulbs. The medial preoptic area (MPOA) is key to expression of consummatory sexual behavior. However, lesions of MPOA fail to prevent preference for an estrous female, suggesting that other brain regions contribute to steroidal effects on sexual motivation. The medial amygdaloid nucleus (Me) is a logical site in this regard. Me receives efferents from the olfactory bulbs, and has abundant steroid receptors. We have shown that testosterone in Me stimulates sexual activity in castrated males. Aims 2B and 2C will use lesions and hormone stimulation to determine the role of Me and MPOA in steroid facilitation of chemosensory responsiveness. These studies have relevance to understanding sexual motivation, and the significance of steroid hormones in appetitive sexual behavior.

描述（由申请人提供）：像大多数哺乳动物一样，性腺类固醇激素对于男性仓鼠的交配至关重要。 cast割也减少了性动机，这是通过对阴道气味的兴趣和对女性的偏爱来衡量的。但是，了解对开胃性行为的类固醇刺激机制是有限的。这是拟议研究的总体目标。仓鼠是研究性动机的重要模型，因为交配取决于化学感应提示。雄性仓鼠在交配前对女性进行了广泛的肛门生态研究，去除嗅球可取消对雌性女性的交配和偏爱。特定目标1中的研究将研究cast割和类固醇替代的影响对雌性仓鼠阴道分泌（FHV）的检测和反应性，以确定cast割的雄性是否可以检测FHV，以及传达对FHV响应的类固醇信号。避免条件的味道将用于评估化学感应检测（AIM 1A），而AIMS 1B和1C将使用FHV的操作响应来评估化学感应响应能力。特定的目标2将确定脑途径的荷尔蒙控制化学感应反应能力。 AIM 2A将研究嗅觉粘膜和/或呕吐器官中奖励化学感应线索的转导。 AIMS 2B和2C将集中在嗅球的中心靶标上。内侧前区域（MPOA）是表达完整性行为的关键。但是，MPOA的病变无法防止偏爱女性的偏爱，这表明其他大脑区域对性动机产生了类固醇的影响。在这方面，内侧杏仁核核（ME）是逻辑部位。我从嗅球中收到传出，并具有丰富的类固醇受体。我们已经表明，我中的睾丸激素会刺激cast割男性的性活动。 AIMS 2B和2C将使用病变和激素刺激来确定ME和MPOA在类固醇促进化学感应反应性中的作用。这些研究与理解性动机以及类固醇激素在食欲行为中的意义相关。