Piezoelectric QCM for Dental Caries Research

用于龋齿研究的压电 QCM

• 批准号: 6852213
• 负责人: FENGXIA QI
• 金额: $ 11.34万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-28 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6852213
• 关键词: Streptococcus mutans; adult human (21+); bacterial genetics; biofilm; biomedical equipment; biomedical equipment development; clinical research; communicable disease diagnosis; confocal scanning microscopy; dental caries; dentistry; diagnostic tests; fluid flow; gene mutation; human subject; immunologic assay /test; measurement; microorganism culture; molecular weight; oral bacteria; patient oriented research; saliva; technology /technique development

DESCRIPTION: Piezoelectric quartz crystal microbalance (QCM) is a powerful tool to measure mass accretion on surfaces in the nanogram range. In the past 10 years, it has found more and more applications in a variety of biological studies from protein-protein interaction, DNA hybridization, surface attachment and biofilm formation, to detection of pathogens in food, water, and urine samples. Nevertheless, this powerful technique has not been applied in Dentistry. In this exploratory research application, investigators from UCLA School of Dentistry (UCLA) and Chemical and Environmental Engineering Department of University of California-Riverside (UCR) are partnering to introduce this technology to Dentistry by developing an integrated system combining QCM with fluidic chamber adaptable to laser scanning confocal microscopy (LSCM). We posit that this integrated system will allow for real-time measurement of biomass accretion with simultaneous analysis of structural development during biofilm formation. It will also allow for rapid, sensitive, and specific detection of oral pathogens with simultaneous image confirmation. Two Specific Aims are proposed for this study. Aim 1 is to develop a LSCM compatible, integrated QCM/fluidic chamber system for oral biofilm studies. Aim 2 is to apply QCM for rapid detection and quantification of S. mutans in mixed cultures. The outcome of this study will be the novel development and application of the QCM technology in Dentistry, which will result in acquisition of new knowledge in Dental biofilm research and development of a novel diagnostic tool that has the potential to be developed into a chair side device for oral pathogen detection.

描述：压电石英晶体微量平衡（QCM）是测量纳米图范围表面上质量积聚的强大工具。在过去的10年中，它在从蛋白质蛋白质相互作用，DNA杂交，表面附着和生物膜形成到食物，水和尿液样品中病原体的各种生物学研究中发现了越来越多的应用。然而，这种强大的技术尚未应用于牙科。在此探索性研究应用程序中，加州大学洛杉矶分校牙科学院（UCLA）和加州大学河滨大学（UCR）的化学与环境工程系的研究人员通过开发将QCM与流体室与激光扫描的共扫描共胶体显微镜（LSCM）相结合，从而将该技术引入牙科。我们认为，该集成系统将允许对生物量积聚进行实时测量，并同时分析生物膜形成过程中结构发育。它还可以同时确认对口腔病原体的快速，敏感和特定检测。为这项研究提出了两个具体目标。目标1是开发用于口服生物膜研究的LSCM兼容的QCM/流体室系统。 AIM 2是应用QCM以快速检测和定量混合培养物中的突变体。这项研究的结果将是QCM技术在牙科中的新开发和应用，这将导致牙科生物膜研究和开发新知识的新知识的新知识，从而有可能将其开发到口腔病原体检测的椅子侧装置中。