Computational analysis of protein/membrane interactions

蛋白质/膜相互作用的计算分析

• 批准号: 6782644
• 负责人: DIANA MURRAY
• 金额: $ 26.27万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-15 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6782644
• 关键词: bioinformatics; biophysics; cell membrane; hydropathy; informatics; intermolecular interaction; ionic bond; membrane fusion; membrane proteins; molecular dynamics; phosphatidylinositols; phospholipase A2; phospholipids; physical model; protein binding; protein protein interaction; protein sequence; protein structure function; protein transport

DESCRIPTION (provided by applicant): A fundamental question in cell biology is how the recruitment of proteins to cellular membranes is achieved and regulated. The long term objective of the proposed research is to characterize the structural and energetic basis for the binding of peripheral membrane proteins to phospholipid membranes and, in turn, to better understand the biophysical basis of membrane-mediated events in cells. The overall strategy is based on two distinct computational approaches: 1) the calculation of the physical interactions between proteins and membranes, and 2) bioinformatics tools for sequence analysis, structure comparison and structure prediction. By studying different membrane-interacting proteins in parallel, two hypotheses will be tested: 1) that two physical factors-electrostatics and hydrophobicity-are the major determinants of membrane binding, and 2) that these physical factors are manifested as patterns in sequence, structure and biophysical characteristics that can be used to predict membrane targeting potential. The first specific aim is to describe how nonspecific electrostatic and hydrophobic interactions mediate the wide range of membrane binding behaviors exhibited by secreted phospholipases A2. The second specific aim is to understand the role of electrostatic interactions in the calcium-dependent and independent membrane binding of C2 domains. The third specific aim is to develop structural models for phosphoinositides, an important class of signaling lipids. The fourth specific aim is to determine the energetic basis of both the specific and non-specific interactions of pleckstrin homology (PH) domains with membranes containing phosphoinositides. The computational results will be used in the design and interpretation of experiments through collaborations with experimental groups and will lead to rules that can be used to detect membrane targeting motifs in proteins. Secreted phospholipases A2 have been implicated in inflammation, and C2 and PH domain-containing proteins involved in phosphoinositide signaling have been implicated in oncogenesis; these proteins require membrane association for their function. Thus, a detailed understanding of the molecular mechanisms underlying the control of membrane association would facilitate the rational design of drugs that inhibit membrane binding.

描述（由申请人提供）：细胞生物学的一个基本问题是如何实现和调节蛋白质向细胞膜的募集。拟议研究的长期目标是表征外周膜蛋白与磷脂膜结合的结构和能量基础，进而更好地了解细胞中膜介导事件的生物物理基础。总体策略基于两种不同的计算方法：1）计算蛋白质和膜之间的物理相互作用，2）用于序列分析、结构比较和结构预测的生物信息学工具。通过并行研究不同的膜相互作用蛋白，将测试两个假设：1）静电和疏水性这两个物理因素是膜结合的主要决定因素，2）这些物理因素表现为序列、结构的模式和可用于预测膜靶向潜力的生物物理特征。第一个具体目标是描述非特异性静电和疏水相互作用如何介导分泌型磷脂酶 A2 表现出的广泛膜结合行为。第二个具体目标是了解静电相互作用在 C2 结构域的钙依赖性和独立膜结合中的作用。第三个具体目标是开发磷酸肌醇（一类重要的信号脂质）的结构模型。第四个具体目标是确定 pleckstrin 同源 (PH) 结构域与含有磷酸肌醇的膜的特异性和非特异性相互作用的能量基础。计算结果将通过与实验组的合作用于实验的设计和解释，并将产生可用于检测蛋白质中膜靶向基序的规则。分泌的磷脂酶 A2 与炎症有关，而参与磷酸肌醇信号传导的包含 C2 和 PH 结构域的蛋白质与肿瘤发生有关；这些蛋白质需要膜结合才能发挥其功能。因此，详细了解控制膜结合的分子机制将有助于合理设计抑制膜结合的药物。