SPIN-LOCK IMAGING OF CARTILAGE

软骨自旋锁成像

• 批准号: 6620111
• 负责人: RAVINDER REEDY
• 金额: $ 11.89万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-15 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6620111
• 关键词: articular cartilage; biochemistry; bioimaging /biomedical imaging; cartilage; cartilage disorder; collagen; cow; early diagnosis; extracellular matrix; histology; magnetic resonance imaging; method development; musculoskeletal disorder diagnosis; noninvasive diagnosis; nuclear magnetic resonance spectroscopy; osteoarthritis; protein degradation; protein protein interaction; proteoglycan; tissue /cell culture

DESCRIPTION (provided by applicant): OA is a major cause of morbidity in the population over 50 and affects more than 40 million Americans. Although evidence implicates cartilage degeneration as the primary cause for OA, no cure exists as yet. Current treatments relieve symptoms but do not inhibit disease progression. However, if the disease is detected in early stages then appropriate therapeutic intervention may be possible. Currently, there are no noninvasive methods to detect early biochemical changes in cartilage. This lack of noninvasive diagnostic methods also hampered the research in the development of potential chondroprotective agents. To develop sensitive, noninvasive diagnostic tools that target early degenerative changes, one needs to understand the structural and biochemical changes that occur during degeneration and correlate these properties to measurable parameters obtained by a noninvasive method. In this application we propose to exploit proton magnetic resonance (MR) relaxation under spin-locking condition to develop a novel MR technique to monitor structural and biochemical changes in cartilage that occur during early OA. Both spectroscopic and imaging measurements will be performed on cartilage tissue models, normal bovine cartilage, on enzymatically degraded bovine cartilage (which are subjected to selective degradation of varying degrees of proteoglycans (PG) or collagen to mimic the structural and biochemical changes that occur in OA). The results obtained from the extracellular matrix (ECM) model systems will enable one to determine contributions from different components of ECM to the observed relaxation and dispersion behavior, and underlying mechanism. The data from the selective enzymatic degradation studies will provide the signal changes and contrast in spin-lock-weighted images and will help in developing optimal imaging parameters to measure changes induced by specific macromolecular degradation. Since the measured MR properties rely not only on tissue contrast, but are also dependent on structural and biochemical changes, this proposed research will aid in the development of a sensitive non-invasive technique for early diagnosis of OA as well as aid in the development/evaluation of potential chondroprotective drugs and treatment therapies.

描述（由申请人提供）： OA 是儿童发病的主要原因 人口超过 50 岁，影响超过 4000 万美国人。 虽然 有证据表明软骨退变是 OA 的主要原因，但没有 治愈方法目前还存在。 目前的治疗可以缓解症状，但不能抑制 疾病进展。 然而，如果疾病在早期被发现，那么 适当的治疗干预是可能的。 目前，没有 检测软骨早期生化变化的非侵入性方法。 这 缺乏无创诊断方法也阻碍了该领域的研究 开发潜在的软骨保护剂。 培养敏感、 人们需要针对早期退行性变化的无创诊断工具 了解在此过程中发生的结构和生化变化 退化并将这些属性与获得的可测量参数相关联 通过非侵入性方法。 在此应用中，我们建议利用质子磁共振 (MR) 自旋锁定条件下的弛豫，开发一种新颖的磁共振技术 监测期间发生的软骨结构和生化变化 早期OA。 光谱和成像测量将在 软骨组织模型，正常牛软骨，酶降解 牛软骨（经过不同的选择性降解 蛋白聚糖（PG）或胶原蛋白的程度来模拟结构和 OA 中发生的生化变化）。 从得到的结果 细胞外基质（ECM）模型系统将使人们能够确定 ECM 的不同成分对观察到的松弛和 分散行为和潜在机制。 数据来自选择性 酶促降解研究将提供信号变化和对比 自旋锁加权图像将有助于开发最佳成像 测量特定大分子降解引起的变化的参数。 由于测量的 MR 特性不仅依赖于组织对比度，而且还依赖于组织对比度。 这项拟议的研究也依赖于结构和生化变化 将有助于开发一种敏感的非侵入性技术，用于早期治疗 OA 的诊断以及帮助开发/评估潜力 软骨保护药物和治疗疗法。