RNASE BASED SELF/NONSELF DISCRIMINATION MECHANISM

基于RNA酶的自/非自歧视机制

• 批准号: 6703633
• 负责人: Peter E. Dowd
• 金额: $ 2.35万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6703633
• 关键词: angiosperms; confocal scanning microscopy; corn; endoribonucleases; gene mutation; microscopy; plant genetics; plant growth /development; plant morphology; pollen; protein localization; transposon /insertion element

The overall goal of this project is to understand how pistil S-Rnase of Petunia inflata discriminate between self pollen and non-self pollen during self-incompatibility interactions. Since the use of RNases as recognition molecules is unique to this type of self-incompatibility, this study will uncover a new model for cellular recognition in biological systems. Furthermore, a number of medically important proteins have been shown to be RNases, e.g., angiogenin which is involved in the vascularization of tumor cells, and two major proteins in the secretory granules of cytotoxic eosinophils which play a role in the immune defense system. Hence, the study of S-Rnase will also expand the scope of the understanding of the biological functions of RNases. The specific aims are 1) to use transposon insertion mutagenesis to identify the so far elusive pollen component of self-incompatibility in order to understand how S- RNases discriminate between self and non-self pollen; 2) to introduce S- Rnase/GFP (green fluorescence protein) fusion constructs into transgenic plants to study whether rejection of self pollen results from allele- specific uptake of S-RNases into pollen tubes.

该项目的总体目标是了解开枪S-RNase如何 矮牵牛侵蚀区分自花和非自我花粉 在自我兼容性相互作用中。由于使用RNass 识别分子是这种类型的自我兼容性所独有的， 研究将发现一个新的生物学细胞识别模型 系统。此外，许多医学上重要的蛋白质已经 被证明是RNass，例如，涉及的血管蛋白 肿瘤细胞的血管化，分泌中的两个主要蛋白质 细胞毒性嗜酸性粒细胞的颗粒，在免疫防御中起作用 系统。因此，S-RNase的研究也将扩大 理解RNass的生物学功能。具体目的是 1）使用转座子插入诱变来识别到目前为止难以捉摸的 自我兼容性的花粉成分，以了解s- rnases区分自我和非自我花粉； 2）引入S- RNase/GFP（绿色荧光蛋白）融合构建到转基因 植物研究是否拒绝自给等位基因 - S-RNass对花粉管的特定摄取。