RNA Polymerase Sigma Factors in Streptococcus pyogenes

化脓性链球菌中的 RNA 聚合酶 Sigma 因子

• 批准号: 6699340
• 负责人: Charles P. Moran
• 金额: $ 26.6万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-15 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6699340
• 关键词: DNA directed RNA polymerase; Streptococcus pyogenes; bacterial genetics; biological signal transduction; disease /disorder model; gene expression; gene mutation; genetic promoter element; genetic regulation; host organism interaction; laboratory mouse; nuclear runoff assay; site directed mutagenesis; skin infection; transcription factor; virulence

Streptococcus pyogenes (the group A streptococcus or GAS) is an important and common human pathogen. The diseases it causes range from self-limiting skin and throat infections, with, however, the potential for serious sequelae including rheumatic heart disease, acute glomerulonephritis, and possibly pediatric neuropsychological disorders, to severe invasive diseases like myositis and streptococcal toxic shock syndrome. Since single strains of the GAS seem to be able to cause most or all of these diseases, regulation of the expression of GAS genes in response to specific environmental differences within the host is probably key in determining the course of the infectious process. We propose here a new approach to learn more about control of gene expression in the GAS by the characterization of a new RNA polymerase (RNAP) secondary sigma factor needed to transcribe genes under different conditions and the characterization of the genes it regulates.

化脓性链球菌（A组链球菌或气体）是一种重要且常见的人类病原体。 它引起的疾病范围从自限性皮肤和喉咙感染范围，但是，包括风湿性心脏病，急性肾小球肾炎以及可能的儿科神经心理障碍，包括严重的后遗症，到严重的肌炎和肌炎造成性疾病等严重的侵入性疾病。 由于气体的单菌株似乎能够引起大多数或全部这些疾病，因此对宿主内特定环境差异的响应来调节气体基因的表达可能是确定传染过程过程的关键。 我们在这里提出了一种新的方法，可以通过表征在不同条件下转录基因以及其调节的基因表征的新的RNA聚合酶（RNAP）次级sigma因子来了解对气体中基因表达的控制。