Mitochondrial Affect on Olfactory Neuron Output

线粒体对嗅觉神经元输出的影响

• 批准号: 6739238
• 负责人: LINAS A BUNTINAS
• 金额: $ 4.64万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6739238
• 关键词: Rana; action potentials; adenosinetriphosphatase; caffeine; calcium flux; chemoreceptors; electrophysiology; immunocytochemistry; intracellular; membrane potentials; mitochondria; olfactory nerve; postdoctoral investigator; potassium channel; ryanodine; voltage /patch clamp

DESCRIPTION (provided by applicant): Because of the spatiotemporal uptake and then subsequent release of calcium by mitochondria, they have been hypothesized to act as memory storage devices. The focus of this project is to determine the possible role of mitochondria in shaping calcium transients and action potential generation in olfactory receptor neurons (ORNs). Whether mitochondrial activity is important for conveying past stimulation of the neuron by affecting neuronal output will be tested. The specific aims are: 1) to determine if mitochondria are important for transient calcium regulation in ORNs and 2) to determine if mitochondrial calcium uptake will influence action potential generation in ORNs. Imaging intracellular calcium, perforated and loose patch electrophysiology are the techniques that will be used to test these hypotheses. Mitochondrial activity will be perturbed and any effects will be observed and quantified. The results gained from this project will further our knowledge of the function of intracellular calcium stores in ORNs and may have implications for potential mechanisms of olfactory coding.

描述（由申请人提供）：由于时空吸收，然后通过线粒体释放钙，因此假设它们充当记忆存储设备。该项目的重点是确定线粒体在嗅觉受体神经元（ORNS）中塑造钙瞬变和动作电位产生中的可能作用。线粒体活性对于通过影响神经元输出传达神经元的过去刺激很重要。具体目的是：1）确定线粒体对于ORNS中的瞬时钙调节以及2）确定线粒体钙的摄取是否会影响ORN的动作电位产生。成像细胞内钙，穿孔和松散的斑块电生理学是用于检验这些假设的技术。线粒体活性将受到干扰，并将观察和定量任何效果。从该项目中获得的结果将进一步我们对ORN中细胞内钙存储功能的功能的了解，并可能对嗅觉编码的潜在机制有影响。