TWO DIMENSIONAL IR W/ N H VIBRATION OF PEPTIDES

肽的二维红外光谱 / N H 振动

• 批准号: 6480808
• 负责人: ROBIN Main HOCHSTRASSER
• 金额: $ 15.58万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6480808
• 关键词: biological products; biomaterials; biomedical equipment development; biomedical resource; proteins; technology /technique

The N-H vibrational modes of peptides at ca. 3( are essentially localized oscillators. They undergo Fermi resonance with N-H bends. The anharmonicity of these N-H vibrations allows them to be seen in the third order nonlinear response and hence in two-dimensional spectroscopy. Since the modes are local the assembly of peptide N-H vibrators can be considered to be a set of N coupled oscillators. The 2D experiments can yield couplings between the various oscillators. The couplings can be used to establish the three-dimensional structure of the peptide. The data obtained in these experiments will be complimentary to those found for amide couplings. With both sets of couplings the determination of peptide structure will be greatly enabled. The goal is top obtain structures at high time resolution and data acquisition rate. The research underway involves the construction of a 3( based nonlinear IR spectrometer. Three methods will be possible with this new apparatus (1) pump/probe where the two dimensions arise from a Fabry-Perot interferometer setting and the array detector dispersion (2) transient grating, which uses three beams (3) spectrally resolved heterodyned photon echo which utilizes two time delays.

肽的 N-H 振动模式。 3(本质上是 局域振荡器。 它们经历 N-H 弯曲的费米共振。 这些 N-H 振动的非和谐性使它们能够在 三阶非线性响应，因此在二维中 光谱学。 由于模式是局部的，因此肽 N-H 的组装 振动器可以被认为是一组 N 个耦合的振荡器。 这 二维实验可以产生各种振荡器之间的耦合。 联轴器可用于建立三维结构 肽。 这些实验中获得的数据将是 与酰胺联轴器的互补。 与两组 耦合肽结构的测定将大大 已启用。 目标是在高时间分辨率下获得最佳结构 和数据采集率。 正在进行的研究涉及 构建基于 3( 的非线性红外光谱仪。三种方法 使用这种新装置（1）泵/探头将成为可能，其中两个 尺寸由法布里-珀罗干涉仪设置和 阵列探测器色散（2）瞬态光栅，它使用了三个 光束 (3) 光谱分辨外差光子回波，利用 两次时间延误。