Novel multi-spectral illumination for microscopy

用于显微镜的新型多光谱照明

• 批准号: 6695056
• 负责人: DAVID J ZAHNISER
• 金额: $ 10万
• 依托单位: DIAGNOSTIC VISION CORPORATION
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2004-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6695056
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; computer program /software; computer system design /evaluation; electron optics; fluorescence microscopy; light emission; light microscopy; semiconduction; spectrometry; technology /technique development; video microscopy

DESCRIPTION (provided by applicant): Conventional microscope bulbs burn out, degrade, and produce a great deal of unnecessary light and heat. For fluorescence microscopy and imaging, only monochromatic, narrow band spectra are generally required, yet with conventional microscopy light sources a significant amount of light and heat is discarded. Solid state, Light Emitting Diode (LED) illumination can provide an illumination source that is well controlled, will not degrade over time, and offers some unique advantages for multi-spectral narrow band illumination. In Phase I of this research grant, we will demonstrate that 1) white multi-die LED illumination modules can be used for general purpose microscope illumination, 2) single and dual wavelength multi-die LED illumination modules can be used for quantitative imaging, and 3) that multi-die LED illumination modules can be used for fluorescence microscopy. The modules being prototyped consists of multiple tiny LED dies (not the large discrete LEDs known in everyday uses) on a single substrate in a group about the size of a standard tungsten halogen bulb filament. In Phase II commercial prototypes will be developed, along with control hardware and software to adjust intensity, choose colors, and allow strobing of the LEDs. With the large number of microscopes in use for medicine and biology, materials science, and in the semiconductor industry, the potential market for these innovative light sources is immense.

描述（由申请人提供）： 常规的显微镜灯泡燃烧，降解并产生大量不必要的光线和热量。对于荧光显微镜和成像，通常只需要单色，狭窄的带光谱，但是传统的显微镜光源可丢弃大量的光和热量。固态，发光二极管（LED）照明可以提供一个受良好控制的照明源，不会随着时间的推移而降级，并为多光谱窄带照明提供了一些独特的优势。在该研究赠款的第一阶段中，我们将证明1）白色多-DIE LED照明模块可用于通用显微镜照明，2）单个和双波长多-DIE LED LED照明模块可用于定量成像，3）可用于荧光显微镜的多-DIE LED照明模块可用于荧光显微镜。原型的模块由一个小组中的单个底物上的多个微小的LED模具（不是大的离散LED）组成，涉及标准钨卤素灯泡丝的大小。将开发在II期商业原型中，以及控制硬件和软件，以调整强度，选择颜色并允许LED的轴承。随着大量用于医学和生物学，材料科学以及在半导体行业的显微镜，这些创新光源的潜在市场是巨大的。