BAC Library Production /Distribuion for 'Healthy people

BAC 图书馆为“健康人”制作/分发

• 批准号: 6895958
• 负责人: ROD A WING
• 金额: $ 10万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6895958
• 关键词: animal genetic material tag; artificial chromosomes; biomedical resource; biotechnology; cooperative study; genetic library; human genetic material tag; molecular biology information system; nucleic acid hybridization; technology /technique development; tissue resource /registry

DESCRIPTION: (provided by applicant) Cloning large DNA fragments using the bacterial artificial chromosome (BAC) library system has revolutionized and accelerated our ability to physically map, positionally clone and sequence whole genomes. Due to the difficulty in cloning large DNA fragments, only a handful of laboratories have been able to produce deep-coverage large insert libraries on a routine basis. This bottleneck has resulted in a limited number of BAC libraries that are available to the research community that can be used for gene discovery, survey sequencing and comparative genome studies. To increase the world?s BAC library construction capacity and reduce costs, the NIH has posted a request for applications (RFA.HG-Ol-002) entitled "BAC Library Production" which is the subject of this application. The specific aims of our thee-year proposal are: BAC Library Production: Develop BAC library construction capacity to construct 36 human genome size ( about3000 Mb) 10X genome equivalent BAC libraries. In year 1, we will produce 6 libraries at $0.50/clone, in year 2, 12 libraries will be produced at $0.375/clone and in year three 18 libraries will he produced at $0.25/clone. The initial average insert size will hover between 130 - 150 kb and we will strive to increase our average insert sizes to 170 - 200 kb in subsequent years. Libraries, filters and clones will be distributed through our CUGI BAC/EST Resource Center on a cost recovery basis (www.genome.clemson.edu). BAC Library Research and Development: We will systematically develop technologies to optimize and streamline BAC library construction procedures in order to reduce the cost of making libraries while maintaining quality and insert size. Also, technologies will be developed to routinely achieve insert sizes of 160kb or greater. In addition, a robust tool kit will be established to produce high-quality megabase-size DNA from animals. This will include the development of a "BIG DNA To-Go" kit to produce high-quality megabase-size DNA on location where the lab needing a library will provide the tissue(s) required to make the library. Bioinformatics Support: A laboratory information management/bar coding system will be developed to aid in tracking library production and distribution and a robust online BAC library ordering system and database will also be developed.

描述：（由申请人提供）使用 细菌人工染色体（BAC）文库系统发生了革命性的变化， 加速了我们物理绘图、定位克隆和测序的能力 整个基因组。由于克隆大DNA片段非常困难，目前仅 少数实验室已经能够生产深度覆盖的大插入物 日常图书馆。这个瓶颈导致了数量有限 可供研究界使用的 BAC 库 用于基因发现、调查测序和比较基因组研究。到 提高全球BAC文库建设能力并降低成本， NIH 已发布题为“BAC 图书馆”的申请请求 (RFA.HG-Ol-002) 生产”是本申请的主题。 我们三年提案的具体目标是： BAC图书馆建设：发展BAC图书馆建设能力建设 36 个人类基因组大小（约 3000 Mb）10X 基因组等效 BAC 文库。在 第 1 年，我们将以 0.50 美元/克隆的价格生产 6 个文库，第 2 年，我们将生产 12 个文库 将以 0.375 美元/克隆的价格生产，第三年将有 18 个文库 生产价格为 0.25 美元/克隆。初始平均插入尺寸将徘徊在 130 之间 - 150 kb，我们将努力将平均插入大小增加到 170 - 200 kb 在随后的几年中。库、过滤器和克隆将被分发 在成本回收的基础上通过我们的 CUGI BAC/EST 资源中心 （www.genome.clemson.edu）。 BAC图书馆研究与发展：我们将系统地发展 优化和简化 BAC 文库构建程序的技术 为了降低图书馆的成本，同时保持质量和 插入尺寸。此外，还将开发技术以常规地实现插入 大小为 160kb 或更大。此外，还将建立一个强大的工具包 从动物中生产高质量的兆碱基 DNA。这将包括 开发“BIG DNA To-Go”试剂盒以生产高质量的兆碱基 DNA 位于需要图书馆的实验室将提供所需组织的位置 制作图书馆。 生物信息学支持：实验室信息管理/条形码系统 将开发以帮助跟踪图书馆的制作和分发以及 还将开发强大的在线 BAC 图书馆订购系统和数据库。