Effects of Acupuncture on Gastric Motility in Rats

针刺对大鼠胃动力的影响

• 批准号: 6666615
• 负责人: Toku Takahashi
• 金额: $ 19.25万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6666615
• 关键词: acetylcholine; acupuncture /acupressure; alternative medicine; diabetes mellitus; dorsal motor nucleus; efferent nerve; electrodes; gene expression; hyperglycemia; immunocytochemistry; intestine obstruction; laboratory rat; microdialysis; neurotransmitter transport; parasympathetic nervous system; postoperative complications; protooncogene; stomach emptying; stomach motility /pressure; sympathetic nervous system

DESCRIPTION (provided by applicant): Acupuncture has been used to treat gastrointestinal (GI) symptoms in China for more than 3,000 years. However, the mechanism of beneficial effects of acupuncture still remains unknown. Our recent studies have shown that acupuncture to the abdomen caused a transient relaxation of the stomach, while the acupuncture to the hind limb caused a transient contraction in thiobutabarbital (Inactin)-anesthetized rats. Gastric relaxations induced by acupuncture to the abdomen were abolished by guanethidine, propranolol, hexamethonium, splanchnic ganglenectomy, but not by vagotomy. In contrast, gastric contractions induced by acupuncture to the hind limb were abolished by atropine, hexamethonium and vagotomy, while splanchnic ganglenectomy and guanethidine had no effects. Both of acupuncture-induced gastric relaxations and contractions were abolished by spino-medullary transection, but not by pontomedullary transection, suggesting the mediation via the brain stem. It is suggested that gastric relaxations and contractions induced by acupuncture are mediated via the somato-sympathetic and somato-parasympathetic neural pathway, respectively. We will study whether acupuncture to the hind limb stimulates c-Fos expression at the dorsal motor nucleus of vagi (DMV), neural activity of vagal efferent and ACh release from the gastric wall. Acupuncture of the lower extremity has been used to treat the patients with anorexia and postoperative ileus in China. Our preliminary study demonstrated that acupuncture to the hind limb potentiates gastric contractions and accelerates solid gastric emptying in conscious rats. It has been demonstrated that gastric emptying is delayed in diabetic rats and that the origin of the delayed gastric emptying was implied to be because of impaired vagal tone. We will study whether acupuncture improves impaired gastric emptying in diabetic rats. Although the mechanism of postoperative ileus still remains unclear, the possibility of reduced vagal tone and increased sympathetic tone has been suggested. We will study whether acupuncture shortens the period of postoperative ileus in rats. This study would provide beneficial information for patients with gastroparesis and postoperative ileus.

描述（由申请人提供）：针灸已用于治疗中国的胃肠道（GI）症状已有3000多年了。但是，针灸有益作用的机制仍然未知。我们最近的研究表明，腹部针灸引起了胃的短暂松弛，而对后肢的针灸引起了硫代省（Inactin）触及的大鼠的短暂收缩。针灸诱导的腹部胃松弛被鸟苷，普萘洛尔，六甲基甲基盐，闪烁腺神经节切除术废除，但没有通过迷走神经术。相比之下，针灸引起的后肢诱导的胃收缩被阿托品，六甲基和迷走神经切开术废除，而闪烁的神经节切除术和鸟苷没有影响。针灸诱导的胃松弛和收缩都被Spino-Mustullary的横向取消，但不是通过庞然大物的横断来取消，这表明通过脑干进行了介导。建议通过针灸引起的胃松弛和收缩分别通过索马托 - 交感神经和索马托 - 交感神经神经途径介导。我们将研究针对后肢的针灸是否在Vagi（DMV）的背运动核（DMV），迷走传递的神经活性和ACH从胃壁释放的神经活动中刺激C-FOS表达。下肢的针灸已被用来治疗中国患有厌食症和术后肠胃疾病的患者。我们的初步研究表明，后肢针灸会增强胃收缩并加速有意识的大鼠的固体胃排空。已经证明，糖尿病大鼠胃排空延迟，并且延迟胃排空的起源暗示是由于迷走神经张力受损。我们将研究针灸是否改善了糖尿病大鼠胃排空受损。尽管术后回肠的机制仍然不清楚，但已经提出了迷走神经音调和同情性语调增加的可能性。我们将研究针灸是否缩短了大鼠术后肠结构的时期。这项研究将为胃轻瘫和术后肠胃疾病患者提供有益的信息。