Core--Human neurobiology

核心--人类神经生物学

• 批准号: 6480792
• 负责人: Victoria Arango
• 金额: $ 17.72万
• 依托单位: NEW YORK STATE PSYCHIATRIC INSTITUTE DBA RESEARCH FOUNDATION FOR MENTAL HYGIENE, INC
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6480792
• 关键词: biomedical facility; cryopreservation; human subject; human tissue; medical records; neuropathology; postmortem; suicide; tissue resource /registry

The Human Neurobiology Core of the CNNMD: The Neurobiology of Suicidal Behavior maintains the Clinical Neuroscience Brain Bank. This involves collection of brain samples and full toxicological, neuropathological and psychiatric characterization of all subjects. All tissues are then sectioned for the various protocols and a series of intercalated sets of glass mounted sections at various coronal levels are kept in storage. Similarly, fixed brainstems are sectioned and stored in cryoprotectant, which allows for various cytoarchitectonic, immunocytochemical and immunoautoradiographic protocols to be systematically performed. The Brain Bank of the Human Neurobiology Core provides all the tissue to the investigators in Project 2 of the Conte Center, as well as other investigators whose R01s are dependent on the tissue from the Bran Bank. It also sends tissue to the Clinical Laboratory Core for toxicological screening of all samples, for genotyping (candidate genes) and the microarray assays of gene expression proposed in the Clinical Laboratory Core. The Human Neurobiology Core provides standardized protocols for all histological, radioligand and immunocytochemical assays proposed including those proposed in Project 1.

CNNMD 的人类神经生物学核心：自杀行为的神经生物学维护着临床神经科学脑库。这涉及收集所有受试者的大脑样本以及完整的毒理学、神经病理学和精神病学特征。然后根据不同的方案对所有组织进行切片，并在不同的冠状水平上保存一系列插入的玻璃安装切片组。同样，固定的脑干被切片并储存在冷冻保护剂中，这使得可以系统地执行各种细胞结构、免疫细胞化学和免疫放射自显影方案。人类神经生物学核心脑库向 Conte 中心项目 2 的研究人员以及 R01 依赖于麸皮库组织的其他研究人员提供所有组织。它还将组织发送到临床实验室核心，对所有样本进行毒理学筛查、基因分型（候选基因）以及临床实验室核心提出的基因表达微阵列分析。人类神经生物学核心为所有提出的组织学、放射性配体和免疫细胞化学检测（包括项目 1 中提出的检测）提供标准化方案。