GENOME RESOURCE BANKING OF RARE FELID POPULATIONS

稀有猫科动物种群的基因组资源库

• 批准号: 6436138
• 负责人: WILLIAM F SWANSON
• 金额: $ 12.67万
• 依托单位: CINCINNATI ZOO AND BOTANICAL GARDENS
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6436138
• 关键词: biopsy; cats; cryopreservation; disease /disorder model; embryo /fetus preservation; embryo /fetus tissue /cell culture; genetic markers; genome; growth media; in vitro fertilization; molecular biology information system; molecular cloning; mucopolysaccharidosis; nuclear transfer; polymerase chain reaction; technology /technique development; tissue resource /registry

DESCRIPTION (Provided by applicant): Domestic cats are valuable research models for the study of genetic disease in humans, providing critical insight into pathology and treatment options, including emerging gene therapies. However, maintenance of cat models can be expensive and challenging due to disease manifestations that interfere with normal reproduction. Genome resource banking, in conjunction with ART, offers a powerful alternative method for research model management and preservation. In addition, this technology may benefit conservation of the world's 36 biologically-imperiled wild cat species. The usefulness of this approach ultimately depends upon applying effective methods for embryo production, cryopreservation, and embryo transfer (ET). In the proposed resource-related research grant, our primary goal is to establish a functional genome resource bank for propagating, managing, and preserving cat disease models and other rare felids. Specific aims include investigating the potential of somatic cell cloning in cats while improving current embryo culture, cryopreservation and transfer methods for more efficient production of offspring, and then applying these techniques to propagation and preservation of fourteen feline models of human hereditary disease and two endangered nondomestic felid species. Initial studies will investigate somatic cell nuclear transfer, embryo culture requirements, alternative cryopreservation and recipient synchronization protocols, and embryo biopsy and diagnostic testing methods. Applied studies will examine the use of embryo cryopreservation and transfer for propagating mucopolysaccharidosis-affected kittens within clinical therapy trials and the feasibility of using somatic cell cloning, embryo cryopreservation and transfer for propagation of other disease models and nondomestic felids. Potential benefits include more productive use of research models, improving animal welfare, expanding usefulness of underutilized models, improving logistics and providing long-term preservation of valuable felid populations.

描述（申请人提供）：家猫是宝贵的研究 研究人类遗传疾病的模型，提供关键的见解 进入病理和治疗方案，包括新兴基因疗法。 但是，由于猫模型的维护可能是昂贵而挑战的 干扰正常繁殖的疾病表现。基因组 资源银行与艺术结合提供了强大的替代方案 研究模型管理和保存方法。此外，这 技术可能会受益于世界36个生物学刺激的保护 野猫物种。这种方法的有用性最终取决于 应用有效的方法来生产胚胎，冷冻保存和胚胎 转移（ET）。在拟议的与资源相关的研究赠款中，我们的主要 目标是建立一个功能性基因组资源库来繁殖， 管理并保存猫病模型和其他罕见的罪犯。具体的 目的包括研究猫中体细胞克隆的潜力 改善当前的胚胎培养，冷冻保存和转移方法 更有效地生产后代，然后将这些技术应用于 人类遗传的14个猫科动物模型的传播和保存 疾病和两种濒临灭绝的非遗传性felid物种。最初的研究将 研究体细胞核转移，胚胎培养需求， 替代性冷冻保存和受体同步协议，以及 胚胎活检和诊断测试方法。应用研究将检查 胚胎冷冻保存和转移的使用 临床治疗试验中受粘二糖的影响小猫和 使用体细胞克隆，胚胎冷冻保存和 传播其他疾病模型和非遗传性罪犯的转移。 潜在的好处包括更有生产力的研究模型，改善动物福利，扩大未充分利用模型的实用性，改善 物流并提供长期保存宝贵的FELID人群。