CORE--HYBRIDOMA/CELL CULTURE/IMMUNE MONITORING

核心--杂交瘤/细胞培养/免疫监测

• 批准号: 6592795
• 负责人: HARRY D BEAR
• 金额: $ 4.12万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-16 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6592795
• 关键词: antibody formation; biomedical facility; hybridomas; monoclonal antibody; tissue /cell culture

The Hybridoma/Cell Culture/Immune Monitoring Shared Resource of the MCC has been active since 1983. Initially, this core laboratory was established for the sole purpose of creating hybridomas and producing monoclonal antibodies for cancer research scientists at VCU. This technology has been critical for several different lines of investigation, including identification and purification of cancer-critical for several different lines of investigation, including identification of purification of cancer-related proteins and localization of these proteins and other molecules in cells and tissues. Over the past decade, as monoclonal antibodies and hybridoma cell lines have become more available from commercial sources, the need to create new hybridoma has decreased somewhat. Simultaneously, this shared resource has expanded its role to include production of monoclonal antibodies, large scale culture of cell lines other than hybridomas, growth of large numbers of lymphocytes for adoptive immunotherapy, mycoplasma testing of cell lines of all types, harvest and preparation of blood samples for correlative studies associated with clinical trials, and harvest and preparation of blood and lymphocyte samples for immunologic monitoring of endpoints associated with clinical immunotherapy trials. As with the original hybridoma production services, all of these functions serve cancer research investigators for whom it would be cumbersome and inefficient to establish the same capabilities in each of their separate laboratories. While preference is given to funded Cancer Center researchers, all VCU scientists are able to access the facility's services. Funding of this shared resource will be obtained from the CCSG and from a system of user fees designed to recover cost of supplies, while still providing a very cost- efficient service to research scientists at VCU and the Cancer Center.

自1983年以来，MCC的杂交瘤/细胞培养/免疫监测共享资源一直活跃。最初，该核心实验室的建立是为了创建杂交瘤并生产VCU癌症研究科学家的单克隆抗体的唯一目的。这项技术对于几种不同的研究行为至关重要，包括鉴定和纯化癌症的几种不同研究线，包括鉴定与癌症相关蛋白的纯化以及这些蛋白质的定位以及细胞和组织中的其他分子。在过去的十年中，随着单克隆抗体和杂交瘤细胞系的商业来源已获得更多的可用性，创建新杂交瘤的需求有所下降。同时，该共享资源扩大了其作用，包括生产单克隆抗体的产生，除杂交瘤以外的其他细胞系的大规模培养，大量用于收养免疫疗法的淋巴细胞的增长，对所有类型的细胞系的支原体测试，用于血液的细胞系，以及与临床试验的较血液制备和制备有关的临床试验，并制备了临床试验，并制备了临床试验，并将其制备。与临床免疫疗法试验相关的终点的免疫学监测。与最初的杂交瘤生产服务一样，所有这些功能都服务于癌症研究研究者，在他们的每个独立实验室中建立相同的功能是繁琐且效率低下的。尽管优先考虑资助的癌症中心研究人员，但所有VCU科学家都可以访问该设施的服务。该共享资源的资金将从CCSG和旨在收回供应成本的用户费用系统中获得，同时仍然为VCU和癌症中心的研究科学家提供非常有效的服务。