CORE--HIGH RESOLUTION MICROSCOPY

核心——高分辨率显微镜

• 批准号: 6605460
• 负责人: KENT T KEYSER
• 金额: $ 19.59万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-02 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6605460
• 关键词: bioimaging /biomedical imaging; biomedical equipment resource; biomedical facility; confocal scanning microscopy; digital imaging

Description: (Applicant's Description) The detection and imaging of molecules in biological systems is an increasingly important tool in biomedical research. Confocal microscopy has become a leading technology for this purpose. In 1998, Dr. Kent Keyser was the recipient of an NIH NCRR Shared Instrument Grant, a UAB Health Services Foundation award, and start-up funds from the UAB Office of the Provost, the Chairs of Departments in both the Medical and Academic Divisions of UAB, and Center Directors. These funds were used to establish a new facility, the High Resolution Imaging Facility. This imaging resource is equipped with two Leica Confocal Imaging Spectrophotometer TCS SP units. One is configured for multiphoton excitation with a Coherent MIRA Ti: Sa ultrafast laser with output selectable between 690-990 nanometers This permits imaging of living cells and tissues with minimal phototoxicity, and of structures 200-400 micrometers from the tissue surface. The SP scan head provides an individual spectrophotometer for each detector channel, allowing selection of specific portions of the emission spectrum for delivery to the detector. The result is higher fluorescence yield, reduced crosstalk, and improved image quality. The Facility also includes an Olympus Fluoview CLSM with fiber launched Krypton ion and Argon ion lasers, conventional fluorescence and DIC microscopy equipment, and work stations equipped with digital deconvolution software, output and image storage devices. In addition, through a cooperative agreement with the Birmingham Veteran?s Administration Medical Center, Cancer Center members will have access to a Leica TCS SP unit equipped with a UV laser and UV corrected optics. All three Leica instruments are equipped with either three or four detector channels, a transmitted light channel, and Argon ion, Krypton ion and Helium: Neon lasers. These systems are able to image up to three fluorophores simultaneously and are capable of obtaining DIC images as well. The software packages offer three-dimensional reconstruction, time-lapse imaging, ratio imaging, physiology packages, and morphometry routines. The Facility will offer Comprehensive Cancer Center members access to a state-of-the-art imaging facility with a full range of instrumentation and the expertise to use these resources productively.

描述：（申请人的描述）分子的检测和成像 在生物系统中，生物医学中越来越重要的工具 研究。共聚焦显微镜已成为领先的技术 目的。 1998年，肯特·基瑟（Kent Keyser）博士成为NIH NCRR共享工具的接受者 UAB Health Services Foundation奖和UAB的启动资金Grant 教务长办公室，医疗和部门主席 UAB和中心董事的学术部门。这些资金习惯了 建立一个新的设施，即高分辨率成像设施。这个成像 资源配备了两个Leica共聚焦成像分光光度计TCS SP 单位。一个使用连贯的mira ti配置了多光子激发： SA超快激光器，可在690-990纳米之间选择输出激光 允许对活细胞和组织的成像，并具有最小的光毒性和 从组织表面的200-400微米结构。 SP扫描头 为每个检测器通道提供一个单独的分光光度计，允许 选择发射光谱的特定部分以交付到 探测器。结果是更高的荧光产量，串扰降低，并且 改进的图像质量。该设施还包括Olympus Fluoview CLSM 随着纤维发射的k离子和氩离子激光器，传统 荧光和DIC显微镜设备，以及配备的工作站 数字反卷积软件，输出和图像存储设备。在 补充，通过与伯明翰资深人士的合作协议 癌症中心的管理医疗中心将有权进入 配备有紫外线和紫外线校正光学的Leica TCS SP单元。这三个 Leica仪器配备了三个或四个检测器通道 透射光通道和氩离子，k离子和氦：霓虹灯激光器。 这些系统能够同时最多图像三个荧光团图像 也能够获得DIC图像。软件包提供 三维重建，延时成像，比率成像， 生理软件包和形态计量法规。该设施将提供 全面的癌症中心成员访问最先进的成像 设施具有全范围的仪器和使用这些工具的专业知识 有效的资源。