CORE--ISLET CORE

核心--胰岛核心

• 批准号: 6564346
• 负责人: Gordon Campbell Weir
• 金额: $ 21.13万
• 依托单位: JOSLIN DIABETES CENTER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564346
• 关键词: animal tissue; biomedical facility; laboratory mouse; laboratory rat; pancreatic islets; swine

Description (taken directly from the application): The objectives and Specific Aims are to provide mouse, rat, and porcine islet tissue to the participants of the PPG for their in vitro studies on immune rejection mechanisms and islet function, for gene transfer experiments, and for transplantation. In addition, the techniques of islet cell transplantation will be taught to investigators and their lab members as required. Background: This PPG is focused upon islet transplantation, with the expectation that porcine islets will be transplanted into humans with IDDM. Because of the serious shortage of human islet tissue, it will probably be necessary to use islets from other species. For a variety of reasons, including ready availability, pigs seem particularly well suited for xenografting. Therefore, both adult porcine islets and porcine neonatal pancreatic cell clusters (NPCCs) will be made available to the participants of the PPG. NPCCs seem particularly promising because they are hardy, have impressive capacity for growth and differentiation, and seem particularly well suited for genetic modification. Rat and mouse islets will also be supplied to the four Projects. Although the goal of this work is to transplant genetically modified pig islets into primates, a large amount of background work must first be carried out with rodent islets, which have proved to be the most widely used models for the study of islet transplantation immunology, as well as islet physiology and cell biology.

描述（直接取自应用程序）：目标和 具体目标是向参与者提供小鼠、大鼠和猪的胰岛组织 PPG 的免疫排斥机制和胰岛体外研究 功能、基因转移实验和移植。在 此外，还将教授胰岛细胞移植技术 根据需要，研究人员及其实验室成员。 背景：本 PPG 专注于胰岛移植， 期待将猪胰岛移植到人体中 IDDM。由于人体胰岛组织严重不足， 可能需要使用其他物种的胰岛。对于各种 原因包括现成的供应情况，猪似乎特别适合 用于异种移植。因此，成年猪胰岛和猪胰岛 新生儿胰腺细胞簇（NPCC）将提供给 PPG 的参与者。 NPCC 似乎特别有前途，因为它们 很坚强，具有令人印象深刻的成长和分化能力，并且 似乎特别适合基因改造。大鼠和小鼠 小岛也将供应给这四个项目。虽然目标是 这项工作是将转基因猪胰岛移植到灵长类动物体内， 大量的背景工作首先必须用啮齿类动物来进行 胰岛，已被证明是该研究中使用最广泛的模型 胰岛移植免疫学，以及胰岛生理学和细胞学 生物学。