CORE--GENETIC HYPERCALCIURIC STONE FORMING RATS

核心--遗传性高钙结石形成大鼠

• 批准号: 6600911
• 负责人: DAVID A BUSHINSKY
• 金额: $ 10.78万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6600911
• 关键词: animal colony; biomedical facility; hypercalciuria; laboratory rat; nephrolithiasis

This core breeds, screens and maintains the genetic hypercalciuric stone forming (GHS) rats. Using a standard protocol each GHS rat is screened for hypercalciuria and the most severely hypercalciuric rats are used to breed the next generation. Currently we have carried out this breeding and screening for 51 generations (as of January 1999) and every rat from GHS parents is hypercalciuric. The GHS rats excrete approximately 9-10 times as much urinary calcium as controls. This core breeds and supplies GHS rats to the Projects and Cores in this Program Project as outlined in the proposal. As needed, rats are placed on matched high and low calcium and phosphate diets and appropriate urine collections are performed.

该核心繁殖，筛选和维持遗传性高钙石（GHS）大鼠。使用标准方案，将每只GHS大鼠筛选为高钙尿症，最严重的高钙尿大鼠用于繁殖下一代。目前，我们已经进行了51代（截至1999年1月）进行了这种繁殖和筛查，而GHS父母的每只老鼠都是过度定量的。 GHS大鼠排出的尿钙是对照的9-10倍。该核心将GHS大鼠在该计划项目的项目和核心中繁殖，如提案中所述。根据需要，将大鼠放在匹配的高钙和磷酸盐饮食上，并进行适当的尿液收集。