CORE--MOUSE

核心--鼠标

• 批准号: 6563892
• 负责人: XUNXIANG DU
• 金额: $ 22.01万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563892
• 关键词: NOD mouse; SCID mouse; animal breeding; animal colony; biomedical facility; genetic manipulation; genetically modified animals; microinjections; plasmids

The Mouse Core is formulated to provide specialized mice to the investigators of this program. The Mouse Core will provide: 1) transgenic mice; or 2) NOD/SCID mice for human-mouse xenografts. The Core utilizes equipment and expertise already in place in the Hermen B Wells Center for Pediatric Research, including two microinjection microscopes, 8 rodent rooms, a Cesium irradiator and specialized HEPA-filtered air mouse cage racks which house the NOD/SCID mice. The core will receive DNA plasmids from investigators and will derive pups from up to 5 injection sessions/construct. Tall DNA will be provided to the investigators for confirmation of transgene presence. The Mouse Core will maintain adequate numbers of breeding mice for each transgene to provide investigators experimental mice in a timely fashion. The NOD/SCID facility is maintained with entry restrictions. The Mouse Core will provide both housing and experimental manipulation serve for each investigator to minimize the risk of colony contamination. All animals in this core are housed under the supervision of the Laboratory Animal Resource Center, an AAALAC-approved facility.

鼠标芯被配制为向专门的小鼠提供 该计划的调查人员。鼠标核心将提供：1）转基因 小鼠；或2）对人类异种移植的点头/SCID小鼠。核心使用 设备和专业知识已经在Hermen B Wells中心 小儿研究，包括两个显微镜，8个啮齿动物 房间，铯辐照器和专门的HEPA过滤空气鼠标 容纳点头/SCID小鼠的架子。核心将接收DNA质粒 来自调查人员，并将从5次注射中得出幼崽 会议/构造。将向调查人员提供高的DNA 确认转基因存在。鼠标核心将保持足够的 每个转基因为研究人员提供的育种小鼠数量 及时的实验老鼠。保持点数/SCID设施 带有进入限制。鼠标核心将同时提供住房和 实验性操纵可用于每个研究者，以最大程度地降低风险 殖民地污染。该核心中的所有动物均被安置在 AAALAC批准的实验室动物资源中心的监督 设施。