Core--Molecular virology

核心--分子病毒学

• 批准号: 6659987
• 负责人: David Richard Gretch
• 金额: $ 21.22万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6659987
• 关键词: biomedical facility; cooperative study; genetic strain; hepatitis C virus; serology /serodiagnosis; tissue /cell culture; virus RNA; virus replication

Description (adapted from the application). The Molecular Virology Core will have three distinct functions to support research on the human hepatocyte cell culture model of HCV replication and liver cell injury. The three functions of the Molecular Virology Core are: 1) to provide HCV RNA from infectious clone sources and viremic sera from infected patient sources for use in infectivity studies; 2) to characterize HCV replication in the human hepatocyte model by quantitative assessment of HCV genome and antigenome production; and 3) track propagation of HCV quasispecies variants in hepatocyte culture by heteroduplex mobility analysis and nucleotide sequencing. These functions are also components of the other sections of the application, and in this regard the Molecular Virology Core will be a service facility. In addition, several key hypotheses on HCV infectivity and viral determinants of hepatocellular injury will be explored.

描述（根据应用程序改编）。分子病毒学核心将 具有三个不同的功能来支持对人肝细胞细胞的研究 HCV复制和肝细胞损伤的培养模型。这三个功能 分子病毒学核心是：1）从传染性克隆提供HCV RNA 来自感染的患者来源的来源和病毒血清用于感染性 研究； 2）通过通过人类肝细胞模型中的HCV复制来表征 HCV基因组和抗基因组产生的定量评估； 3）轨道 Heteroduplex的HCV准植物变体的传播 迁移率分析和核苷酸测序。 这些功能也是 应用程序的其他部分的组成部分，在这方面 分子病毒学核心将是服务设施。 另外，几个钥匙 关于肝细胞损伤的HCV感染性和病毒决定因素的假设 将被探索。