NMR Console Upgrade and Cryoprobe

NMR 控制台升级和冷冻探针

• 批准号: 6440236
• 负责人: PETER B. MOORE
• 金额: $ 50万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440236
• 关键词: biomedical equipment purchase; nuclear magnetic resonance spectroscopy; structural biology

We request funding so that the console of the Varian Unity 500 MHz NMR spectrometer housed in the Yale Department of Molecular Biophysics and Biochemistry Bass Center can be replaced, and a cryoprobe purchased for that spectrometer. The Varian Inova console requested will replace a Unity console that was built ca. 1990, and was purchased by Yale, second hand, in 1995. Inova consoles have a more robust computer interface that requires fewer reboots, and that executes pulses, power level, phase changes and frequency changes more efficiently than Unity consoles. This upgrade should result in significantly improved implementations of pulse sequences. In addition, Inova consoles are compatible with Varian newest software, SPINCAD, which do3es not run on Unity or Unity plus systems. Thus this upgrade should ensure the usefulness of our 500 MHZ spectrometer for years to come. Last, but not least, replacement of the console in question will reduce the amount of instrument time lost for repair, which has become considerable because the Unity console is reaching the end of its useful life. The cryoprobe requested has recently been developed by Varian. Its major attraction is its high sensitivity, relative to room temperature probes. The sensitivity of the 500 MHz spectrometer equipped with a cryoprobe should be double the sensitivity observed with the best room temperature probe now available on the Yale 800 MHz spectrometer. There are several structural biology projects under consideration that demand this sensitivity because the molecules of interest aggregate at the concentrations required when standard probes are used.

我们要求提供资金，以便可以更换耶鲁大学分子生物物理学和生物化学贝司中心的Varian Unity 500 MHz NMR光谱仪的控制台，并为该光谱仪购买了冷冻探针。所请求的Varian Inova控制台将替换构建的统一控制台。 1990年，由二手耶鲁（Yale）于1995年购买。Inova控制台具有更强大的计算机接口，需要更少的重新启动，并且比Unity Consoles更有效地执行脉冲，功率水平，相变和频率更改。该升级应导致脉冲序列的实现显着改善。此外，Inova控制台与Varian最新软件Spincad兼容，该软件不在Unity或Unity Plus系统上运行。因此，此升级应确保未来几年我们500 MHz光谱仪的有用性。最后但并非最不重要的一点是，相关控制台的替换将减少维修损失的仪器时间，这已经变得很大，因为统一控制台已达到其使用寿命的终结。瓦里安（Varian）最近开发了要求的冷冻探针。它的主要吸引力是相对于室温探针，其高灵敏度。配备有冷冻探针的500 MHz光谱仪的敏感性应是耶鲁800 MHz光谱仪上现在可用的最佳室温探针观察到的灵敏度的两倍。正在考虑的几个结构生物学项目需要这种敏感性，因为目的分子在使用标准探针时所需的浓度汇总。