LC/quadrupole ion trap mass spectrometer

LC/四极杆离子阱质谱仪

• 批准号: 6441125
• 负责人: BARRY M WILLARDSON
• 金额: $ 24.59万
• 依托单位: BRIGHAM YOUNG UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6441125
• 关键词: AIDS; DNA binding protein; RNA binding protein; angiotensin receptor; apoptosis; biological signal transduction; biomedical equipment purchase; calmodulin dependent protein kinase; carcinogenesis; dendritic cells; electrospray ionization mass spectrometry; enzyme substrate; gas chromatography mass spectrometry; liquid chromatography mass spectrometry; mass spectrometry; molecular site; noninsulin dependent diabetes mellitus; nonvisual photoreceptor; pathologic process; phosphorylation; posttranslational modifications; prostaglandin endoperoxide synthase; protein structure function; receptor binding; receptor expression; transcription factor

The elucidation of covalent protein structure is a valuable tool in every field of biomedical research. This goal, the identification of protein sequence and post-translational modifications, is uniquely facilitated through mass spectrometry. Among mass spectrometers designed to analyze biological samples, quadruple ion trap instruments offer superior sensitivity and high quality fragmentation data, as well as coupling to liquid chromatography. For these reasons, we propose to obtain a LCQ DECA investigators from across campus will make use of this instrument, including a Major Users Group consisting of seven NIH RO1 grantees. The instrument will be used in the following ongoing funded research: Dr. BM Willardson will identity phosphorylation sites, interacting proteins, and transcription factors in his investigations of the roles of the phosducin family of G-protein regulators in modulating signal transduction. Dr. MB Andrus will identify receptor-binding drug candidates from solution-phase natural product-like libraries synthesized in his lab. Dr. TS Elton will identify both DNA and RNA binding proteins that play roles in regulating the expression of the angiotensin receptor. Dr. PB Savage will identify and characterize Lipid A-binding anti-microbial candidates from combinatorial libraries of cationic steroid triamides synthesized in his lab. Dr. DL Simmons will investigate the post-translational modifications of cyclooxygenase 2 and nucleobindin, as part of his research into the roles of these proteins in carcinogenesis and apoptosis. Dr. GF Burton will identify proteins implicated in the function of follicular dendritic cells in AIDS pathogenesis. And Dr. WW Winder will identify post-translational modifications, kinase targets, and transcription factors as part of his ongoing research into the physiology of exercise and type 2 diabetes. The LCQ mass spectrometer enables these investigations which would not be feasible otherwise.

共价蛋白质结构的阐明是生物医学研究各个领域的宝贵工具。质谱法独特地促进了蛋白质序列和翻译后修饰的鉴定这一目标。在设计用于分析生物样品的质谱仪中，四重离子阱仪器提供卓越的灵敏度和高质量的碎片数据，以及与液相色谱的耦合。出于这些原因，我们建议获得 LCQ DECA 整个校园的研究人员将使用该工具，包括由 7 名 NIH RO1 受资助者组成的主要用户组。该仪器将用于以下正在进行的资助研究：BM Willardson 博士将在研究 G 蛋白调节剂的磷酸蛋白家族在调节信号转导中的作用时鉴定磷酸化位点、相互作用的蛋白质和转录因子。 MB Andrus 博士将从他实验室合成的溶液相天然产物样库中鉴定受体结合候选药物。 TS Elton 博士将鉴定在调节血管紧张素受体表达中发挥作用的 DNA 和 RNA 结合蛋白。 PB Savage 博士将从其实验室合成的阳离子类固醇三酰胺组合库中鉴定并表征脂质 A 结合抗微生物候选药物。 DL Simmons 博士将研究环氧合酶 2 和核结合蛋白的翻译后修饰，作为他研究这些蛋白质在癌发生和细胞凋亡中的作用的一部分。 GF Burton 博士将鉴定与艾滋病发病机制中滤泡树突状细胞功能有关的蛋白质。 WW Winder 博士将确定翻译后修饰、激酶靶标和转录因子，作为他正在进行的运动和 2 型糖尿病生理学研究的一部分。 LCQ 质谱仪使这些研究成为可能，否则这些研究是不可行的。