PURCHASE OF A VARIAN INOVA 600 MHz NMR SPECTROMETER

购买 VARIAN INOVA 600 MHz 核磁共振波谱仪

• 批准号: 6440324
• 负责人: ERIC T. KOOL
• 金额: $ 50万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-15 至 2003-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440324
• 关键词: biomedical equipment purchase; enzyme structure; nuclear magnetic resonance spectroscopy; protein protein interaction; protein structure function; structural biology

We propose to purchase a 600 MHz Varian Inova Nuclear Magnetic Resonance spectrometer for the Chemistry Department at Stanford University. This instrument will enable researchers to determine high precision structures of macromolecules including proteins, nucleic acids, and protein-protein, protein-ligand, and protein-nucleic complexes. The instrument will directly support the research groups of the P.I. and co-P.I.s (representing approximately 80 researchers), and it is expected that they will be the major users, but will also be available to the Department as a whole (approximately 350 researchers). The instrument will be placed in existing space in the NMR lab in the Chemistry Department, and will be maintained by 2 full-time Ph.D. NMR staffpersons and by 5 graduate assistants. The research proposed specifically identifies the need for a devoted high field NMR instrument for biomolecular structure analysis. Such research includes structural studies of enzymes that synthesize natural product antibiotics, anticancer drugs and the interaction with their target enzymes, a polyketide and derivatives involved in apoptosis, protein-protein and protein-ligand interactions involved in immune response, DNA helices involved in DNA replication and DNA repair, and RNA relating to Hepatitis C virus. All of the P.I.s' research programs are currently supported by NIH and most by NSF as well. Little of this work can be accomplished on current equipment of the Chemistry Department, and all four P.I.s have been forced recently to go elsewhere to carry out needed structural studies, hampering their research. The specific instrumental package we propose to purchase includes the Oxford 14.1 T magnet, as well as supershims, two Fullband RF channels with three total RF channels, waveform generators for each channel, a variable temperature unit, pulsed field gradients, a Sun workstation, and a cryo probe.

我们建议为斯坦福大学的化学系购买600 MHz Varian Inova核磁共振光谱仪。 该仪器将使研究人员能够确定包括蛋白质，核酸和蛋白质 - 蛋白质，蛋白质 - 配体和蛋白质核复合物在内的大分子的高精度结构。 该仪器将直接支持P.I.的研究小组和Co-p.i.s（代表大约80位研究人员），预计他们将成为主要用户，但也将为整个部门提供（约350名研究人员）。 该仪器将放置在化学部门NMR实验室的现有空间中，并将由2个全职博士维护。 NMR StaffPersons和5名研究生助理。该研究提出的专门确定了对生物分子结构分析的专用高场NMR仪器的需求。 Such research includes structural studies of enzymes that synthesize natural product antibiotics, anticancer drugs and the interaction with their target enzymes, a polyketide and derivatives involved in apoptosis, protein-protein and protein-ligand interactions involved in immune response, DNA helices involved in DNA replication and DNA repair, and RNA relating to Hepatitis C virus. 所有P.I.S的研究计划目前都得到NIH和NSF的大多数支持。这项工作几乎无法在化学部门的当前设备上完成，最近四个P.I.最近被迫去其他地方进行所需的结构研究，从而阻碍了他们的研究。我们建议购买的特定仪器包包括牛津14.1 t磁铁，以及超湿磁铁，两个带有三个总RF通道的成型型RF通道，每个通道的波形发电机，一个可变温度单位，脉冲现场梯度，Sun Workstation，一个Sun Workstation和一个冷冻探头。