Purchase of 800 MHz NMR Spectrometer

购买800 MHz核磁共振波谱仪

• 批准号: 6441010
• 负责人: ARTHUR PARDI
• 金额: $ 50万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-15 至 2005-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6441010
• 关键词: biomedical equipment purchase; nuclear magnetic resonance spectroscopy; structural biology

A consortium of three Universities: The University of Colorado, Boulder, the University of Colorado Health Science Center (UCHSC) and the University of Utah School of Medicine will purchase an 800 MHZ NMR spectrometer with partial support from the NCRR Shared Instrumentation and NSF Multi-User Biological Instrumentation Program. This field NMR will benefit the research programs of the seven major users (A. Pardi and D.S. Wuttke at Boulder; W.L. Sundquist and D.R. Davis at Utah; and B.K. Bendiak, D.N.M Jones, and M.J. Overduin at UCHSC) and several minor users at the three institutions. Recent developments in TROSY-type experiments allow acquisition of extremely high resolution NMR spectra on much larger macromolecules than was previously possible. These TROSY experiments are optimized at ultra-high magnetic fields, and the requested 800 MHZ NMR will make it possible for the major users to take advantage of these powerful new techniques. The instrument will be housed in the basic science building that is part of the new campus currently being constructed for UCHSC in Denver. The major users currently have access to 500 and 600 MHZ NMR instrumentation so the requested 800 NMR will be used for projects that have a demonstrated need for a higher field, higher resolution and higher sensitivity of the 800 MHz spectrometer. The requested instrument will accelerate new and ongoing research projects in the following areas: - Oligosaccharide Structures and Cell Surface Carbohydrate-Protein Interactions- B.K. Bendiak - Structural Studies of HIV Primer/Template Complex-D.R. Davis - Structural Function Studies of Proteins and Carbohydrates Involved in Disease- D.N.M. Jones - Structures, Interactions and Dynamics of Membrane-Associated Proteins- M.J. Overduin - Structure-Function Studies of RNA-A. Pardi - Structural Studies of HIV-1 Assembly and Budding- W.I. Sundquist - Structural Studies of Biologically Important Molecular Recognition- D.S. Wuttke

由三所大学组成的财团：科罗拉多大学，博尔德大学，科罗拉多大学健康科学中心（UCHSC）和犹他大学医学院将购买800 MHz NMR光谱仪，并在NCRR共享仪器和NSF多用途生物学仪器计划中得到部分支持。该领域的NMR将使七个主要用户的研究计划受益（Boulder的A. Pardi和D.S. Wuttke； W.L. Sundquist和D.R. Davis在犹他州；以及B.K. Bendiak，D.N.M Jones，D.N.M Jones和M.J.在UCHSC上的M.J. Overduin）以及三个机构的几个未成年用户。最新的Trosy型实验的发展允许在比以前更大的大分子上获取极高的分辨率NMR光谱。这些Trosy实验在超高磁场上进行了优化，并且所需的800 MHz NMR将使主要用户有可能利用这些强大的新技术。该乐器将安置在基础科学大楼中，该大楼是目前为丹佛UCHSC建造的新校园的一部分。目前，主要用户可以访问500和600 MHz NMR仪器，因此所请求的800 NMR将用于具有更高领域，更高分辨率和更高灵敏度的项目，对800 MHz光谱仪。所请求的仪器将在以下领域加速新的和正在进行的研究项目： - 寡糖结构和细胞表面碳水化合物 - 蛋​​白质相互作用-B.K。 Bendiak- HIV底漆/模板复合-d.r的结构研究。戴维斯 - 参与疾病的蛋白质和碳水化合物的结构功能研究-D.N.M.琼斯 - 膜相关蛋白质的结构，相互作用和动力学 - M.J.过度 - RNA-A的结构 - 功能研究。 PARDI- HIV-1组装和芽