CORE--CONUS VENOMS RESOURCE

核心--芋螺毒液资源

• 批准号: 6564576
• 负责人: LOURDES J CRUZ
• 金额: $ 14.53万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564576
• 关键词: Gastropoda; animal colony; animal poison; biomedical facility; calcium channel blockers; complementary DNA; conotoxin; cryopreservation; high performance liquid chromatography; laboratory mouse; molecular cloning; neuropeptides; peptide analog; polymerase chain reaction; protein purification; synthetic peptide; tissue resource /registry

The venom resource core established in the first grant period will be expanded to provide a large collection of Conus venoms and to improve the facility to support a highly efficient operation for identification of specific venom components. Conus specimens will be collected from several countries, but most of them will be obtained from various localities of the Philippines, the richest source of Conus species; about 200 of the 500 known species are found in the Philippines. The venom will be processed in the Marine Science Institute of the University of the Philippines and transported to the University of Utah where a system for rapid assay, fractionation and purification has been developed. By the end of the grant period, we anticipate that the venom core facility will have venoms or venom ducts of over 90 species of Conus. Purified natural Conus peptides, synthetic peptides, derivatives and analogs will also be maintained by the core facility. The collection of venom ducts and other tissues from selected Conus species will be used for preparation of cDNA libraries which will be needed to rapidly expand conotoxin sequence databases through cDNA cloning. Furthermore, many thousands of venom ducts will be collected in preparation for increasing our efforts in the enzymology of post-translational modification.

在第一个赠款期间建立的毒液资源核心将是 扩展以提供大量的圆锥毒液并改善 支持高效操作以识别的设施 特定的毒液成分。圆锥标本将从几个 国家，但其中大多数将是从各个地区获得的 菲律宾，最富有的圆锥形物种来源；大约有500个 已知物种在菲律宾发现。毒液将在 菲律宾大学海洋科学学院和 运送到犹他大学，那里有快速测定系统， 分馏和纯化已经开发出来。到赠款结束时 时期，我们预计毒液核心设施将有毒液或 90多种圆锥体的毒液管道。纯化的天然圆锥肽， 合成肽，衍生物和类似物也将由 核心设施。收集毒液管和其他组织 选定的圆锥物种将用于制备cDNA库 将需要快速扩展结合毒素序列数据库需要 通过cDNA克隆。此外，数千种毒管将是 收集以准备增加我们在酶学中的努力的准备 翻译后修改。