CORE--DRUG INTERACTIONS

核心——药物相互作用

基本信息

批准号：
6643655
负责人：
WILLIAM F TRAGER
金额：
$ 25.41万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-08-01 至 2003-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6643655
关键词：
Baculoviridae affinity labeling biomedical facility cryopreservation cytochrome P450 drug interactions enzyme structure gas chromatography mass spectrometry human tissue intestines liver cells mathematical model microspectrophotometry model design /development protein isoforms protein sequence tissue resource /registry

项目摘要

DESCRIPTION (adapted from the application): This PPG has one administrative core element and three scientific core elements (the mass spectrometry unit, the human liver bank unit, and the computational core unit) that have been established to provide shared resources critical to the day-to-day needs of the participating investigators and to the future scientific development of the PPG as a whole. Dr. Trager will direct all components of this core unit and manage the administrative components with support for a budget coordinator. In managing the scientific components, Dr. Trager will be assisted by Dr. Howald, the Technical Director of the Mass Spectrometry Center, Dr. Thummel, the Director of the Human Liver and Intestine Bank (with the aid of an assistant). Dr. Perkins, the Director of the Division of Transplantation in the Department of Surgery (to facilitate the acquisition of samples for the human tissue bank), Dr. Jones, the Director of the Computational Component of the Core Unit, and Dr. Hackett of the Division of Transplantation in the Department of Medicinal Chemistry (who will provide expertise and support in protein biochemistry, protein mass spectrometry, and micro-capillary chromatography). Dr. Kharasch will provide clinical expertise and collaborate on the in vivo studies described in Projects 1 and 4. The mass spectrometry center contains six instruments with different capabilities. These instruments will be used for drug and metabolite determinations, protein mass determinations, and peptide sequencing. This center will be used extensively by all four projects associated with this PPG proposal. The human tissue bank now contains well-characterized frozen samples of 53 human livers and 60 human intestines. These tissues are available to all investigators affiliated with the PPG. The procurement of human livers and intestines will continue throughout the proposed funding period to replace tissue that will be utilized in the four research projects. The computational component of this core will be provided as part of a consortium agreement with the University of Rochester in New York, where Dr. Jones is located along with one full-time and one part-time assistant. Three dimensional active site models are currently being developed as part of the research efforts in Projects 2 and 4, and there is some expectation that other projects in the PPG may eventually draw on this resource. Funds are also requested to buy two major pieces of equipment: an Innova 90-6 argon laser that would be used in photoaffinity labeling experiments in Projects 2 and 4, and a Cary 4 Bio UV-Vis spectrophotometer to be used in the four research projects in this PPG proposal.

描述（改编自应用程序）：此 PPG 有一个管理核心要素和三个科学核心要素（质谱单元、人类肝库单元和计算核心单元）旨在提供对日常需求至关重要的共享资源参与研究人员以及未来的科学发展 PPG 作为一个整体。 Trager 博士将指导该核心单元的所有组成部分并在预算支持下管理行政部分协调员。在管理科学部分时，Trager 博士将由质谱技术总监Howald博士协助中心，人类肝脏和肠道银行主任 Thummel 博士（与助理的帮助）。 Perkins 博士，该部门主任外科移植（以促进获得人体组织库样本），琼斯博士，主任核心单元的计算部分，以及该部门的Hackett博士药物化学系的移植（谁将提供蛋白质生物化学、蛋白质质谱分析等方面的专业知识和支持微毛细管色谱法）。 Kharasch 博士将提供临床专业知识并合作进行项目 1 和 4 中描述的体内研究。质谱中心拥有六台不同类型的仪器能力。这些仪器将用于药物和代谢物测定、蛋白质质量测定和肽测序。这该中心将被与该 PPG 相关的所有四个项目广泛使用提议。人体组织库现在包含 53 份特征明确的冷冻样本人类肝脏和 60 个人类肠道。这些纸巾可供所有人使用隶属于 PPG 的调查员。人类肝脏的采购和 Enters 将在整个拟议资助期间继续进行，以取代将在四个研究项目中使用的组织。该核心的计算组件将作为与纽约罗切斯特大学达成联盟协议，博士。琼斯与一名全职助理和一名兼职助理一起工作。三目前正在开发维度活性位点模型，作为项目 2 和 4 的研究工作，并且有一些期望其他 PPG 中的项目最终可能会利用此资源。还需要资金购买两件主要设备：一台 Innova 90-6 氩激光将用于光亲和标记实验项目 2 和 4，以及 Cary 4 Bio 紫外-可见分光光度计将用于 PPG 提案中的四个研究项目。