Animal Core

动物核心

• 批准号: 6739514
• 负责人: William M Baldwin
• 金额: $ 20.39万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6739514
• 关键词: animal colony; biomedical facility; blood vessel transplantation; heart transplantation; laboratory mouse; laboratory rat; transplantation immunology

DESCRIPTION (provided by applicant): The rat and mouse have been chosen as the central animal models for in vivo and many in vitro studies in this program project. Rats and mice are uniquely suited for these investigations because of the large data base on cardiac transplants and atherosclerosis that the participants in this program project have already accumulated and that are available in the literature. The Animal Core has been functioning for five years as a common resource for in vivo models of AGA. The major benefit of having an Animal Core is the optimized use of animals by sharing material from control and experimental cardiac transplants that are performed with a uniform technical expertise. This has promoted the comparison of vascular lesions that develop in all of the models used by different projects. The role of the animal core in facilitating collaborative use of critical animal models as they are developed. One example of this is the use of various knock out mice. After the importance of iNOS was established in the rejection of B 10.A cardiac allografts to C57BL/6 recipients with iNOS knock outs, this strain combination was expanded for use with eNOS, von Willebrand factor and P-selectin knock outs by Dr. Lowenstein, and Granzyme B knock outs by Dr. Rosen. A uniform method of immunosuppression was adopted (treatment with monoclonal antibodies to CD4) to prevent acute rejection. As a result common controls could be used and comparisons among experimental groups could be made for end points of common interest, such as cellular infiltrates, endothelial injury and release of von Willebrand factor. Animal models of cardiac allografts will continue to be used in all of the proposed projects. Three projects will make use of mice and rats and two projects will use primarily just mice or rats.

描述（由申请人提供）： 在该程序项目中，已选择大鼠和小鼠作为体内和许多体外研究的中心动物模型。大鼠和小鼠非常适合这些研究，因为该计划项目的参与者已经积累了有关心脏移植和动脉粥样硬化的数据基础，并且文献中可以使用。 动物核心已经起作用了五年，是AGA体内模型的共同资源。拥有动物核心的主要好处是通过共享具有统一技术专长的对照和实验性心脏移植的材料来优化动物。这促进了在不同项目使用的所有模型中发展的血管病变的比较。 动物核心在促进关键动物模型的协作中的作用。一个例子是使用各种敲除小鼠。在拒绝B 10.a心脏同种异体移植对C57BL/6受iNOS敲击的受体中的心脏同种异体移植物中建立了重要性之后，该菌株组合被扩展到eNOS，von willebrand因子和p-Selectin fircom和p-Selectin ovck uts a Lowenstein博士。 ，以及Rosen博士的Granzyme B击败。采用了一种均匀的免疫抑制方法（用CD4的单克隆抗体治疗），以防止急性排斥。结果，可以使用共同的控制，并可以在实验组之间进行比较，以终止共同兴趣的终点，例如细胞浸润，内皮损伤和von Willebrand因子的释放。 所有提议的项目都将继续使用心脏同种异体移植的动物模型。三个项目将利用小鼠和大鼠，两个项目将主要使用小鼠或大鼠。