Core--Neuropathology and Cell Culture

核心--神经病理学和细胞培养

• 批准号: 6825333
• 负责人: BARBARA J CRAIN
• 金额: $ 24.73万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6825333
• 关键词: DNA damage; autoradiography; biomedical facility; cerebral ischemia /hypoxia; histology; immunocytochemistry; in situ hybridization; neuropathology; terminal nick end labeling; tissue /cell culture

The Neuropathology/Cell Culture Core (Core B) has a central role in our Program Project. The approach of Core B will be multidisciplinary, using neuropathological and cell culture methods to evaluate mechanisms of ischemic brain injury. There are two main aims. In the first aim, the staff of Core B will be responsible for preparing all histologic sections for studies of focal and global ischemia, including sections processed for infarct volume determination, immunohistochemistry, DNA break staining (TUNEL), and in situ hybridization. The staff will assist in training personnel from Projects 1-5 in the associated data analysis. In the second aim, the staffof Core B will prepare and maintain all primary cell cultures for projects 1, 4, and 5.). Core B staff will also perform different models of induced-toxicity and perform immunocytochemistry, in vitro receptor autoradiography, cell binding assays, and pharmacological treatments on cultured cells. The staff will assist in training personnel from the various Projects in the data analysis associated with the cell cultures.

神经病理学/细胞培养核心（核心B）在我们的程序项目中起着核心作用。核心B的方法将是多学科的，使用神经病理学和细胞培养方法来评估缺血性脑损伤的机制。有两个主要目标。在第一个目的中，核心B的工作人员将负责为局灶性和全球缺血研究的所有组织学部分准备，包括用于梗塞数量确定，免疫组织化学，DNA断裂染色（TUNEL）和原位杂交的部分。工作人员将在相关数据分析中协助项目1-5的培训人员。在第二个目标中，核心b 将为项目1、4和5的所有主要细胞培养准备并维护所有原始细胞培养。核心B员工还将执行不同的诱导毒性模型，并对培养细胞进行免疫细胞化学，体外受体放射自显影，细胞结合测定和药理学治疗。工作人员将协助与细胞培养物相关的数据分析中各个项目的培训人员。