CAVITATION & WOUND HEALING IN SHOCK WAVE LITHOTRIPSY

空化

• 批准号: 6604172
• 负责人: GLENN Michael PREMINGER
• 金额: $ 35.03万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6604172
• 关键词: biomarker; cytokine; diagnosis design /evaluation; iatrogenic disease; inflammation; injury; kidney disorder; lipid peroxides; lithotripsy; microdialysis; noninvasive diagnosis; pathologic process; prostaglandin endoperoxide synthase; swine; wound healing

DESCRIPTION (Adapted from the Applicant's Abstract): Shock wave lithotripsy (SWL) is now used to manage almost 85 percent of all urinary stone patients. Yet, basic and clinical studies have demonstrated that SWL can induce acute and long-term bio-effects at both cellular and tissue levels. Moreover, SWL-induced tissue injury has been postulated to be linked either directly or indirectly with shock wave generated cavitation in vivo. Therefore, the overall goal of this proposed investigation is to assess quantitatively the inertial (? initial) cavitation and subsequent biological consequences generated by SWL in vivo. Two broad approaches will be undertaken: 1) to develop non-invasive techniques for the assessment of SWL-induced cavitation in vivo and its correlation with resultant tissue injury, and 2) to characterize and modulate the host responses in order to decrease inflammation and cell death. Our Specific Aims are as follows: Aim 1. To develop a new cavitation detection system with improved spatial resolution for the assessment of SWL-induced cavitation invivo. Cavitation threshold in different regions of the swine kidney will be determined using a hybrid cavitation detection/shock wave generator system of small focal size ( about 2mm). Aim 2. To examine wound healing in SWL-induced renal injury. Proinflammatory, cytokines, lipid peroxidation levels, and Cox-2 activity will be assessed in microdialysis samples, tissue sections, and blood samples in SWL-treated swine models. The effects of anti-oxidant and anti-inflammatory agents on SWL-induced renal damage will be determined. Aim 3. To assess dose-dependency of SWL-induced tissue injury. Characteristics in acoustic emission (AE) signals associated with SWL-induced bubble oscillations in tissue will be determined under clinically relevant lithotripsy conditions. A dose-dependent variation in the AE signals will be correlated with the corresponding changes in resultant tissue injury. Such a correlation could provide a non-invasive marker for assessing the progression of tissue injury in situ during SWL.

描述（改编自申请人的摘要）：冲击波碎石术 （SWL）现在用于管理所有尿石患者的近85％。 然而，基础研究和临床研究表明，SWL可以诱导急性和 在细胞和组织水平上的长期生物效应。而且，SWL引起的 已经假定组织损伤直接或间接连接 在体内产生冲击波的空化。因此，总体目标 拟议的调查是为了定量评估惯性（？ 最初的）空化和随后的生物学后果由SWL在 体内。将采用两种广泛的方法：1）发展无创 评估SWL诱导的体内空化的技术及 与最终组织损伤相关，2）表征和调节 宿主反应以减少炎症和细胞死亡。我们的 具体目的如下：目标1。开发新的空化检测 具有改进的空间分辨率的系统，用于评估SWL诱导的系统 空化Invivo。猪不同区域的空化阈值 肾脏将使用杂交空化检测/冲击波确定 小焦距（约2mm）的发电机系统。目标2。检查伤口 SWL引起的肾脏损伤中的愈合。促炎，细胞因子，脂质 过氧化水平和COX-2活性将在微透析中评估 经SWL处理的猪模型中的样品，组织切片和血液样品。这 抗氧化剂和抗炎剂对SWL诱导的肾脏的影响 将确定损坏。目标3。评估SWL诱导的剂量依赖性 组织损伤。与声发射（AE）信号相关的特征 在SWL诱导的组织中，将确定在组织中的气泡振荡 临床相关的碎石疗法。剂量依赖性变化 AE信号将与结果的相应变化相关 组织损伤。这样的相关性可以为 评估SWL期间组织损伤的进展。