Antioxidants and oxidative damage in mitochondria

线粒体中的抗氧化剂和氧化损伤

• 批准号: 6445052
• 负责人: CECILIA GIULIVI
• 金额: $ 7.31万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6445052
• 关键词: alpha benzopyrone; antioxidants; chemical conjugate; chemical structure function; chemical substitution; flavonoids; laboratory rat; methylation; mitochondria; nitric oxide; oxidative stress

DESCRIPTION (provided by applicant) The focus of this project resides on the role of small antioxidant molecules on the prevention and/or repair of nitrated proteins in mitochondria. Protein nitration occurs in biological systems exposed to nitric oxide. The addition of a nitro group to a tyrosine residue, yielding 3-nitrotyorosine, has been claimed to be a post-translational modification, part of signal transduction pathways, or a result of "normal" oxidative stress. In this study, the investigators will characterize the mechanism underlying the nitration of proteins in nitric oxide-producing mitochondria, given the important role that these organelles have in the maintenance of cellular ATP, beta-oxidation, heme synthesis, and other functions. The studies will be aimed at understanding the kinetic and structural changes caused by nitration, and how naturally- occurring antioxidants present in fruits and vegetables may prevent/reverse this effect. The antioxidant effect of flavonoids, coumarins, and catechins will be studied in terms hydroxyl substitution, conjugation of rings, and o- methylation of hydroxyl groups to elucidate an association between structure and function.

描述（由申请人提供） 该项目的重点在于抗氧化小分子的作用 关于预防和/或修复线粒体中硝化蛋白质。 蛋白质 硝化发生在暴露于一氧化氮的生物系统中。 补充 将硝基转化为酪氨酸残基，得到 3-硝基酪氨酸 声称是翻译后修饰，信号转导的一部分 途径，或“正常”氧化应激的结果。 在这项研究中， 研究人员将描述硝化的机制 产生一氧化氮的线粒体中的蛋白质，鉴于其重要作用 这些细胞器具有维持细胞 ATP、β-氧化、血红素的作用 合成等功能。 这些研究旨在了解 硝化引起的动力学和结构变化，以及如何自然- 水果和蔬菜中存在的抗氧化剂可能会预防/逆转 这个效果。 类黄酮、香豆素和儿茶素的抗氧化作用 将在羟基取代、环共轭和邻位方面进行研究 羟基的甲基化以阐明结构之间的关联 和功能。