VIRUSES, VIRAL PROTEINS & RECEPTORS: HIV REVERSE TRANSCRIPTASE STRUCT

病毒、病毒蛋白

• 批准号: 6667798
• 负责人: STEPHEN COPLAN HARRISON
• 金额: $ 14.27万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2003-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6667798
• 关键词: AIDS; bioimaging /biomedical imaging; biological products; biomedical resource; immunology; infection; inflammation; lymphatic system; proteins; radiography; structural biology; virus

Inner capsid particles of reovirus and rotavirus. Reovirus cores, particles of about 700
diameter, crystallize in space group F432, a=1249
. The crystals diffract to 3.5
resolution. We have used dual Fuji images plates, mounted in a special cassette on the F1 beamline, to collect data in 0.1 degree oscillation frames. Lower resolution data have been measured using the Quantum 4 detector. We have assembled a relatively complete data set, and we have commenced calculations using cryoEM images to determine initial phases. We have also screened for heavy-atom derivatives. The rotavirus ICPs, similar in size and function, crystallize in space group P212121, a=700
, b=1000
, c=1400
. We have collected a partial high-resolution data set, using dual image plates, as well as a much more complete lower-resolution data set, using the Quantum 4 detector. Human transferrin receptor. We have determined the structure of the transferrin receptor ectodomain to about 3.2
resolution, using data collected from unliganded crystals as well as crystals soaked in SmCl3 and in the platinum compound PIP. The space group is P212121, a = 105, b = 217, c = 364 (8 polypeptide chains per asymmetric unit). The Quantum 4 detector on F1 was important for obtaining sufficiently accurate data. The 70 kDa polypeptide chain folds into three domains, one of which mediates dimerization. Another domain bears a striking and unanticipated similarity to certain metalloproteases. The structure is being refined. Clathrin terminal domain. We have determined the structure of the 50 kDa N-terminal domain of clathrin. There is a globular portion, with a 7-blade beta propeller fold, and a stem-like region based on an alpha-helical zig-zag. HIV reverse transcriptase. We have determined the structure of a template/primer/dNTP complex of HIV reverse transcriptase at 3
resolution, using data collected at F1 with the Quantum 4 detector. The crystals are in space group P212121, a = 78.5, b = 150.0, c = 279.3. The structure reveals aspects of the catalytic mechanism and suggests explanations for the effects of drug resistance mutations. Papillomavirus L1. We have begun studies of crystals of recombinant pentamers of HPV L1. There are several crystal forms, all with very large unit cells.

蠕虫病毒和轮状病毒的内膜颗粒。 葡萄病毒核心， 直径约700的颗粒，在空间群F432中结晶， A = 1249。晶体衍射为3.5分辨率。 我们已经使用过 双富士图像板，安装在F1上的特殊盒式盒子中 梁线，以0.1度振荡框架收集数据。 降低 使用量子4检测器测量了分辨率数据。 我们 已经组装了一个相对完整的数据集，我们已经开始 使用冷冻图像确定初始阶段的计算。 我们有 还筛选了重原子衍生物。 轮状病毒ICP，相似 在大小和功能上，在空间群中结晶P212121，a = 700， B = 1000，C = 1400。我们收集了部分高分辨率数据 设置，使用双图板以及更完整的 低分辨率数据集，使用量子4检测器。 人类 转铁蛋白受体。 我们已经确定了 使用数据，转铁蛋白受体过域域可分辨率约3.2分辨率 从非物体晶体以及浸泡在SMCL3中的晶体收集 并在铂复合PIP中。 空间组为p212121，a = 105，b = 217，C = 364（每个不对称单元8个多肽链）。 这 F1上的量子4检测器对于获得足够 准确的数据。 70 kDa多肽链折叠成三个域， 其中之一介导二聚化。 另一个领域带有惊人的 与某些金属蛋白酶的意外相似性。 这 结构正在完善。 网格蛋白终端结构域。 我们有 确定了网格蛋白的50 kDa N末端结构域的结构。 有一个球状部分，带有7刀片β螺旋桨折叠，一个 基于Alpha螺旋式曲折的茎状区域。 艾滋病毒逆转 转录酶。 我们已经确定了 HIV逆转录酶在3的模板/引物/DNTP复合物 解决方案，使用用量子4检测器在F1收集的数据。 晶体位于空间群中P212121，A = 78.5，B = 150.0，C = 279.3。 该结构揭示了催化机制和 建议解释耐药性突变的影响。 乳头瘤病毒L1。 我们已经开始研究重组晶体 HPV L1的五聚体。 有几种晶体形式，都非常 大型单元。