Calcium Sensing Receptor in Human Airway Epithelium

人体气道上皮中的钙敏感受体

• 批准号: 6644889
• 负责人: PHILLIP E LAMBERTY
• 金额: $ 5.76万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6644889
• 关键词: calcium metabolism; cilium /flagellum motility; clinical research; cystic fibrosis; hormone receptor; human tissue; immunoregulation; ion transport; mucins; postdoctoral investigator; protein localization; receptor; receptor coupling; receptor expression; respiratory epithelium; second messengers

DESCRIPTION (provided by applicant): The sensing receptor (CaSR) is an interesting protein initially characterized in the elucidation of parathyroid hormone release by bovine parathyroid glands. A G-protein coupled receptor, described agonists other than calcium include other polycations (Mg2+, Gd3+), polyamines (spermine, neomycin), and aminoglycosides. It has been described to regulate cellular processes in cell types and tissues not associated with calcium homeostasis. Through some initial studies, using immuno-fluorescence, we have shown expression of the CaR in bronchial epithelia grown in culture from human explants. With confocal microscopy and co-localization with a cytoplasmic mucin, we have localized the protein to reside solely on and near the base of bronchial epithelial cilia. We have confirmed the presence of the CaR in human bronchial epithelia with Western blotting. Given CaSR's intriguing location on the apical surface of ciliated bronchial epithelial tissue, and it's rather unique residence on cilia we reason that this protein will prove to be involved in one or several processes unique to the apical environment-ciliary function, ion transport, and host defense. Specifically, we aim to elucidate a function regarding ciliary beat regulation and ion transport as well as investigate second messenger systems generated via CaSR stimulation.

描述（由申请人提供）：感应受体（CASR）是一种有趣的蛋白质，最初是在牛甲状旁腺释放甲状旁腺激素释放的。 G蛋白偶联受体描述了钙以外的其他激动剂包括其他多阳离子（MG2+，GD3+），多胺（精子，新霉素）和氨基糖苷。已描述为调节与钙稳态无关的细胞类型和组织中的细胞过程。通过一些初步研究，使用免疫荧光，我们显示了汽车在人类外植体培养物中生长的支气管上皮中的表达。通过共聚焦显微镜和与细胞质粘蛋白共定位，我们将蛋白质定位为仅在支气管上皮纤毛的基部和附近。我们已经证实了汽车在人支气管上皮中的存在，并具有蛋白质印迹。考虑到CASR在纤毛支气管上皮组织的顶部表面上的有趣位置，并且在纤毛上是相当独特的住所，我们认为这种蛋白质将被证明参与了一个或几个过程，这是顶端环境纤维功能，离子运输和宿主和宿主独有的一个或几个过程。防御。具体而言，我们旨在阐明有关睫状比调节和离子运输的功能，并研究通过CASR刺激产生的第二质固定系统。