The Identity/Role of IP3 Receptor Associated Proteins

IP3 受体相关蛋白的身份/作用

• 批准号: 6660382
• 负责人: RANDEN LEE PATTERSON
• 金额: $ 4.64万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-16 至 2004-09-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6660382
• 关键词: binding sites; biological signal transduction; calcium flux; calcium indicator; carbohydrate receptor; immunoprecipitation; inositol phosphates; laboratory rat; neurons; neurotransmitter transport; nitric oxide; protein protein interaction; protein structure function; receptor binding; receptor expression; tissue /cell culture; yeast two hybrid system

DESCRIPTION (provided by applicant): Vesicle trafficking of neurotransmitters, neuronal response to nitric oxide, and SOCaE are critical functions in neurons. The InsP3-R may be a crucial regulator of these processes (see sec 30b). Binding partners of the InsP3-R that we identify may modulate such regulation. Recent work in this field has demonstrated that the InsP3-R is a central entity in many Ca2+ signaling pathways in neuronal systems. Identification of the microenvironment of proteins in which the InsP3-R resides will further our knowledge of InsP3-R, and its binding partners involvement within in neuronal signaling pathways. Additionally it will increase our knowledge of how the InsP3-R is able to discriminate between the many pathways that converge on its activity. The main objective of this proposal is to examine the role of the InsP3-R and InsP3-R signaling pathways in neurons. Specifically I intend to identify binding partners for the InsP3-R, and examine their roles in neurotransmitter secretion, SOCaE, and regulation of the InsP3-R itself.

描述（由申请人提供）： 神经递质的囊泡运输，神经元对一氧化氮的反应， 和 SOCaE 是神经元中的关键功能。 InsP3-R 可能是至关重要的 这些过程的监管者（参见第 30b 节）。 InsP3-R 的结合伙伴 我们确定可能会调整此类监管。该领域最近的工作有 证明 InsP3-R 是许多 Ca2+ 信号传导的中心实体 神经系统中的通路。微环境识别 InsP3-R 所在的蛋白质将进一步加深我们对 InsP3-R 的了解， 及其参与神经信号通路的结合伙伴。 此外，它将增加我们对 InsP3-R 如何能够 区分集中于其活动的许多途径。 该提案的主要目标是研究 InsP3-R 的作用和 神经元中的 InsP3-R 信号通路。具体来说，我打算确定 InsP3-R 的结合伴侣，并检查它们在神经递质中的作用 InsP3-R 的分泌、SOCaE 和调节本身。