Role of Nucleus Accumbens GABA in Feeding Behavior

伏核 GABA 在摄食行为中的作用

• 批准号: 6660756
• 负责人: Valerie Gayle Olson
• 金额: $ 4.16万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2004-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6660756
• 关键词: anorexic agent; behavior test; behavioral /social science research tag; behavioral genetics; biological models; gamma aminobutyrate; gene deletion mutation; gene expression; gene targeting; genetically modified animals; immunocytochemistry; laboratory mouse; metabolism; model design /development; neural transmission; nucleus accumbens; nutrient intake activity; nutrition related tag; overeating; phenotype; postdoctoral investigator; transfection /expression vector; weight gain; weight loss

DESCRIPTION: (provided by applicant): The nucleus accumbens is widely implicated in appetitive behaviors, including intake of food and drugs of abuse. It is also known to be critical to certain forms of learning. Further, distinct subregions of the nucleus accumbens (NAc) seem to regulate specific appetitive or defensive behaviors, including feeding behavior. The predominant type of neuron in the nucleus accumbens is the GABAergic medium spiny neuron. GABAergic neurotransmission in this region is thought to regulate feeding behavior. We propose to compromise GABA function genetically by creating a conditional GAD67 knockout mouse in which we can eliminate GAD67 in a neuroanatoinically exact manner within the nucleus accumbens. We hypothesize that compromising GABAergic neurotransmission in this region will produce a behavioral phenotype similar to that of blocking excitatory input to this region, or to that of inhibiting neurons in this region, resulting in hyperphagic mouse. We plan to examine these mice behaviorally to assess changes in feeding behavior, weight gain, and metabolism. Together, these integrated, multidisciplinary studies will advance my training as well as elucidate the role of GABAergic transmission in the nucleus accumbens in regulating feeding behavior.

描述：（由申请人提供）：伏隔核广泛 与食欲的行为有关，包括食物和药物的摄入量 虐待。众所周知，它对于某些形式的学习至关重要。更远， 伏隔核（NAC）的不同子区域似乎调节了特定 开胃态或防御行为，包括喂食行为。主要的 伏隔核中神经元的类型是GABA能培养基神经元。 人们认为该地区的GABA能神经传递可以调节饲料 行为。 我们建议通过创建有条件的 GAD67淘汰鼠标，我们可以在神经旁虫中消除GAD67 伏隔核内的精确方式。我们假设妥协 该地区的GABA能神经传递将产生行为表型 类似于阻止对该区域的兴奋性输入或 抑制该区域的神经元，导致倍感小鼠。我们计划 在行为上检查这些小鼠以评估喂养行为的变化，体重 增益和新陈代谢。 这些综合的多学科研究将共同​​提高我的培训 以及阐明GABA能传播在核中的作用 在调节喂养行为方面的a伏人。