CORE--PHENOTYPIC MARKERS

核心——表型标记

• 批准号: 6564847
• 负责人: LAWRENCE W CASTELLANI
• 金额: $ 23.48万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564847
• 关键词: apolipoproteins; biomedical facility; blood chemistry; blood lipid; blood lipoprotein; blood lipoprotein metabolism

A major effort of Core A is to analyze human and mouse plasmas and serum for lipoproteins and their component lipids and apolipoproteins. The lipid analyses employ automated micro-method techniques to determine levels of total cholesterol, HDL* cholesterol, unesterified cholesterol, triacyglycerols, glycerol banks, and unesterified fatty acids. Human plasma LDL and VLDL concentrations including apoB, apoAI, apoAII, and Lp(a). In addition to the measurement of lipids, lipoproteins, and apolipoproteins, the Phenotypic Marker Core also determines levels of several other plasma factors related to lipid/lipoprotein metabolism and atherosclerosis, including plasma concentrations of insulin, glucose, leptin, and the enzymatic activities of lipoprotein lipase and hepatic lipase. All of the assays required to determine the phenotypes proposed in this Program Project are currently in use in our laboratory.

核心A的主要努力是分析脂蛋白及其成分脂质和载脂蛋白的人和小鼠血浆和血清。脂质分析采用自动微生物技术来确定总胆固醇，HDL*胆固醇，未酯化的胆固醇，三乙二醇，甘油库和未酯化的脂肪酸的水平。人血浆LDL和VLDL浓度在内，包括APOB，APOAI，APOAII和LP（A）。除了测量脂质，脂蛋白和载脂蛋白外，表型标记核心还确定了与脂质/脂蛋白代谢和动脉粥样硬化有关的其他几种血浆因子的水平，包括血浆的胰岛素，葡萄糖，葡萄糖，瘦素和lipase lipase lipase lipase lipase lipase lipase lipase lipase lipase lipase lipose lipase lipase lipose lipose lipase and Heps lipase and Heps lipase and Heps lipase lipase lipase lipose ant hep lipase lipase and Hep lipase and Heps and Hepope。确定该计划项目中提出的表型所需的所有测定方法目前都在我们的实验室中使用。