COCCIDIOIDES IMMITIS EVOLUTION AND VACCINE PRODUCTION

球孢子菌的进化和疫苗生产

• 批准号: 6657471
• 负责人: JOHN W. TAYLOR
• 金额: $ 15.71万
• 依托单位: VETERANS MEDICAL RESEARCH FDN/SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6657471
• 关键词: Coccidioides immitis; biochemical evolution; fungal genetics; fungal vaccines; genetic polymorphism; genetic strain; genetic transcription; microorganism culture; microorganism growth; nucleic acid sequence; phenotype; protein sequence; vaccine development

DESCRIPTION (Adapted from application): As evolutionary mycologists, the investigators propose to contribute to the vaccine project by making sure that natural variation in the genotype of the pathogen is accounted for in vaccine development and testing as follows: 1) expand the search to find all genetically differentiated populations or genetically isolated species of C. immitis. Previous studies have focused on clinical isolates from the United States. To search for additional genetic variation, the investigators propose to use the protocols they developed using U.S. clinical isolates on isolates from Mexico, soil, and non-human mammals; 2) use C. immitis isolates representing the genetically distinct groups found in our expanded search to examine nucleotide and amino acid variation in proteins selected for vaccine development. Examination of the genes, and infection of the amino acid sequence, will alert the investigators to potential problems in the use of overly variable genes and proteins; 3) use the same collection of isolates to measure transcription levels for genes of proteins that are candidates for vaccine development. Low levels of transcription may foreshadow problems with vaccine development; 4) provide the same collection of isolates for vaccine testing in mice; and 5) seek phenotypic correlates of genotypic differences in the California and non-California C. immitis species and optimize a genotyping method to encourage clinicians to compare pathogen genotype and disease progress.

描述（根据应用程序改编）：作为进化的真菌学家， 调查人员建议通过确保该疫苗项目为疫苗项目做出贡献 病原体基因型的自然变异是在疫苗中解释的 开发和测试如下：1）扩展搜索以找到所有 遗传学分化的种群或遗传分离的C。 侵入性。先前的研究集中于联合的临床分离株 国家。为了寻找其他遗传变异，研究人员提出了 使用他们使用美国临床分离株制定的方案 来自墨西哥，土壤和非人类哺乳动物； 2）使用C. Immisis分离株 代表我们扩展的搜索中发现的遗传学不同的群体 检查选择用于疫苗的蛋白质的核苷酸和氨基酸变化 发展。检查基因和氨基酸的感染 序列，将提醒调查人员使用的潜在问题 过于变化的基因和蛋白质； 3）使用相同集合的分离株 测量蛋白质基因的转录水平 疫苗开发。较低的转录可能预示了 疫苗开发； 4）提供相同的疫苗分离株 在小鼠中进行测试； 5）寻求基因型差异的表型相关性 加利福尼亚和非加利福尼亚氏炎种类并优化基因分型 鼓励临床医生比较病原体基因型和疾病的方法 进步。